Interdependency of brassinosteroid and auxin signaling in Arabidopsis.
Interdependency of brassinosteroid and auxin signaling in Arabidopsis.
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DOI：
10.1371/journal.pbio.0020258
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发表时间：
2004-09
期刊：
影响因子：
9.8
通讯作者：
Chory J
中科院分区：
文献类型：
--
作者：
Nemhauser JL;Mockler TC;Chory J
生长调节剂如何引发特定环境下的信号是发育生物学中的一个基本问题。在植物中，生长素和油菜素内酯（BRs）都能促进细胞扩张，并且人们曾认为它们通过独立的机制激活这一过程。在这项工作中，我们描述了一个幼苗生长所必需的生长素 - 油菜素内酯共享通路。遗传、生理和基因组分析表明，一条通路的响应需要另一条通路的功能，并且这种相互依赖并不作用于激素生物合成控制层面。生长素水平的升高会使油菜素内酯刺激的生长反应达到饱和，并极大地降低油菜素内酯对基因表达的影响。这两条通路的整合位于BES1和Aux/IAA蛋白的下游，它们是已知的在每种激素下游起作用的最后调控因子，并且很可能直接发生在生长素 - 油菜素内酯靶基因的启动子上。我们开发了一种新方法来识别在每种激素通路以及生长素 - 油菜素内酯共享通路中起作用的潜在调控元件。我们表明，在生长素和油菜素内酯诱导基因的启动子中高度富集的一个元件对两种激素都有响应，并且需要油菜素内酯的生物合成才能正常表达。这项工作从根本上改变了我们对油菜素内酯和生长素信号传导的看法，并描述了一种强大的新方法来识别对特定刺激作出响应所需的调控元件。
尽管细胞扩张需要不同的生长调节剂——生长素和油菜素内酯；但每条通路的响应并非相互独立，而是需要对方。
How growth regulators provoke context-specific signals is a fundamental question in developmental biology. In plants, both auxin and brassinosteroids (BRs) promote cell expansion, and it was thought that they activated this process through independent mechanisms. In this work, we describe a shared auxin:BR pathway required for seedling growth. Genetic, physiological, and genomic analyses demonstrate that response from one pathway requires the function of the other, and that this interdependence does not act at the level of hormone biosynthetic control. Increased auxin levels saturate the BR-stimulated growth response and greatly reduce BR effects on gene expression. Integration of these two pathways is downstream from BES1 and Aux/IAA proteins, the last known regulatory factors acting downstream of each hormone, and is likely to occur directly on the promoters of auxin:BR target genes. We have developed a new approach to identify potential regulatory elements acting in each hormone pathway, as well as in the shared auxin:BR pathway. We show that one element highly overrepresented in the promoters of auxin- and BR-induced genes is responsive to both hormones and requires BR biosynthesis for normal expression. This work fundamentally alters our view of BR and auxin signaling and describes a powerful new approach to identify regulatory elements required for response to specific stimuli. Although distinct sets of growth regulators - auxin and brassinosteroids - are required for cell expansion; rather than being independent signals, the response from each pathway requires the other
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