Clinical significance of CDC25A and CDC25B expression in squamous cell carcinomas of the oesophagus.
Clinical significance of CDC25A and CDC25B expression in squamous cell carcinomas of the oesophagus.
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DOI：
10.1054/bjoc.2001.1934
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发表时间：
2001-08-03
影响因子：
8.8
通讯作者：
Monden M
中科院分区：
文献类型：
--
作者：
Nishioka K;Doki Y;Shiozaki H;Yamamoto H;Tamura S;Yasuda T;Fujiwara Y;Yano M;Miyata H;Kishi K;Nakagawa H;Shamma A;Monden M
CDC 25 A、CDC 25 B和CDC 25 C属于蛋白磷酸酶家族，其在细胞周期的不同点激活细胞周期蛋白依赖性激酶。根据越来越多的证据，CDC 25 A和CDC 25 B似乎具有致癌特性。我们分析了这些表达的免疫组化，蛋白质印迹和RT-PCR在一系列的100例食管鳞状细胞癌。与癌旁细胞相比，CDC 25 A和CDC 25 B在癌细胞胞浆中呈强阳性表达，阳性率分别为46%（46例）和48%（48例）。CDC 25 A和CDC 25 B的表达与其他细胞周期调控分子如cyclin D1、Rb、p16 INK 4、p27 KIP 1和PCNA的表达无明显相关性。CDC 25 A（+）和CDC 25 B（+）在肿瘤浸润深度和淋巴结转移的患者中更常见，而肿瘤大小仅与CDC 25 A表达相关。CDC 25 A（+）患者的术后生存率明显低于CDC 25 A（-）患者，但不受CDC 25 B状态的影响。在51例（51%）病例中观察到CDC 25 A的核定位，而不考虑其胞浆表达，并且与临床病理因素或预后无关。多因素分析显示，在这些生物学和临床病理因素中，只有CDC 25 A状态是一个独立的有意义的预后因素。CDC 25 A可能是食管鳞癌的一个新的预后因子。因此，在细胞周期中的G1检查点的调节可能是重要的食管癌的发生，这也可能涉及许多其他癌基因。© 2001年癌症研究运动http://www.bjcancer.com
CDC25A, CDC25B and CDC25C belong to a family of protein phosphatases which activate the cyclin-dependent kinase at different points of the cell cycle. According to accumulating evidence, CDC25A and CDC25B seem to possess oncogenic properties. We have analysed these expressions by immunohistochemistry, western blot and RT-PCR in a series of 100 patients with squamous cell carcinoma of the oesophagus. When compared with non-cancerous cells, CDC25A and CDC25B were strongly expressed in the cytoplasm of cancer cells, with positive (+) classification in 46% (46 cases) and 48% (48 cases), respectively. There was no significant correlation between CDC25A and CDC25B expression, nor was there any association with the expression of other cell cycle-regulating molecules, including cyclin D1, Rb, p16INK4, p27KIP1 and PCNA (proliferating cell nuclear antigen). CDC25A (+), as well as CDC25B (+), was more frequently found in patients with deeper tumour invasion and lymph node metastasis, while tumour size was correlated only with CDC25A expression. Postoperative survival was significantly poorer for CDC25A (+) patients than CDC25A (–) patients, but was not affected by the CDC25B status. Nuclear localization of CDC25A was observed in 51 cases (51%), regardless of its cytoplasmic expression, and was not associated with clinico-pathological factors or prognosis. Multivariate analysis revealed only the CDC25A status to be an independent significant prognostic factor among these biological and clinico-pathological factors. CDC25A but not CDC25B may be a new prognostic factor for squamous cell carcinoma of the oesophagus. Thus, regulation of the G1 checkpoint in the cell cycle may be important in oesophageal carcinogenesis, which may also involve many other oncogenes. © 2001 Cancer Research Campaign http://www.bjcancer.com
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影响因子：
8.8
作者：
Kang, SH;Bang, YJ;Jong, HS;Seo, JY;Kim, NK;Kim, SJ
通讯作者：
Kim, SJ
DOI：
10.1111/j.1349-7006.1999.tb00795.x
发表时间：
1999-06
期刊：
Japanese journal of cancer research : Gann
影响因子：
--
作者：
Kokunai T;Tamaki N
通讯作者：
Tamaki N
DOI：
10.1073/pnas.87.13.5139
发表时间：
1990-07-01
影响因子：
11.1
作者：
SADHU, K;REED, SI;RUSSELL, P
通讯作者：
RUSSELL, P
影响因子：
64.5
作者：
GALAKTIONOV, K;BEACH, D
通讯作者：
BEACH, D
DOI：
10.1073/pnas.85.1.6
发表时间：
1988-01-01
影响因子：
11.1
作者：
CHRETIEN, S;DUBART, A;ROMEO, PH
通讯作者：
ROMEO, PH