Passive mechanical properties and related proteins change with botulinum neurotoxin A injection of normal skeletal muscle.
Passive mechanical properties and related proteins change with botulinum neurotoxin A injection of normal skeletal muscle.
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DOI：
10.1002/jor.21533
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发表时间：
2012-03
影响因子：
2.8
通讯作者：
Ward, Samuel R.
中科院分区：
文献类型：
--
作者：
Thacker, Bryan E.;Tomiya, Akihito;Hulst, Jonah B.;Suzuki, Kentaro P.;Bremner, Shannon N.;Gastwirt, Randy F.;Greaser, Marion L.;Lieber, Richard L.;Ward, Samuel R.
肉毒杆菌神经毒素A对骨骼肌的被动力学特性的影响尚未研究，但可能在治疗神经肌肉疾病（包括痉挛）方面具有显著影响。对肉毒杆菌神经毒素A处理的大鼠肌肉进行单纤维和纤维束被动力学测试。凝胶电泳法测定肌球蛋白重链和肌联蛋白的组成。使用羟脯氨酸测定法测定肌肉胶原蛋白含量。与对照纤维相比，神经毒素处理的单纤维被动弹性模量降低（53.00 kPa对63.43 kPa）。与对照纤维相比，纤维硬度和松弛肌节长度也减少，肌球蛋白重链组成从更快转变为更慢的亚型。处理后肌联蛋白平均分子量增加1.77%。纤维束被动弹性模量在治疗后增加（168.83 kPa对75.14 kPa）。束刚度也增加，而每质量肌肉组织的胶原蛋白含量增加38%。注射肉毒杆菌神经毒素A对正常肌肉的被动机械特性产生影响，这与在痉挛肌肉中观察到的变化相反。
The effects of botulinum neurotoxin A on the passive mechanical properties of skeletal muscle have not been investigated, but may have significant impact in the treatment of neuromuscular disorders including spasticity. Single fiber and fiber bundle passive mechanical testing was performed on rat muscles treated with botulinum neurotoxin A. Myosin heavy chain and titin composition of single fibers was determined by gel electrophoresis. Muscle collagen content was determined using a hydroxyproline assay. Neurotoxin-treated single fiber passive elastic modulus was reduced compared to control fibers (53.00 kPa versus 63.43 kPa). Fiber stiffness and slack sarcomere length were also reduced compared to control fibers and myosin heavy chain composition shifted from faster to slower isoforms. Average titin molecular weight increased 1.77% after treatment. Fiber bundle passive elastic modulus increased following treatment (168.83 kPa versus 75.14 kPa). Bundle stiffness also increased while collagen content per mass of muscle tissue increased 38%. Injection of botulinum neurotoxin A produces an effect on the passive mechanical properties of normal muscle that is opposite to the changes observed in spastic muscles.
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影响因子：
1.5
作者：
TROTTER, JA;PURSLOW, PP
通讯作者：
PURSLOW, PP
影响因子：
5.1
作者：
Love, SC;Valentine, JP;Chauvel, PJ
通讯作者：
Chauvel, PJ
影响因子：
11
作者：
HESSE, S;LUCKE, D;FRIEDRICH, H
通讯作者：
FRIEDRICH, H
影响因子：
56.9
作者：
WOOD, DS;ZOLLMAN, J;BRANDT, PW
通讯作者：
BRANDT, PW
DOI：
10.1046/j.1365-201x.2001.00848.x
发表时间：
2001-06-01
期刊：
ACTA PHYSIOLOGICA SCANDINAVICA
影响因子：
--
作者：
Ahtikoski, AM;Koskinen, SOA;Takala, TES
通讯作者：
Takala, TES