Structural basis for single-stranded RNA recognition and cleavage by C3PO.
Structural basis for single-stranded RNA recognition and cleavage by C3PO.
复制标题
C3PO 识别和切割单链 RNA 的结构基础
DOI：
10.1093/nar/gkw776
复制
发表时间：
2016-11-02
影响因子：
14.9
通讯作者：
Gan J
中科院分区：
文献类型：
--
作者：
Zhang J;Liu H;Yao Q;Yu X;Chen Y;Cui R;Wu B;Zheng L;Zuo J;Huang Z;Ma J;Gan J
Translin和translin-associated factor-x在真核细胞中是高度保守的;它们可以形成异聚复合物（称为C3 PO）并参与各种核酸代谢途径。在人类和果蝇中，C3 PO切割片段化的siRNA乘客链并促进RNA诱导的沉默复合物（RNA干扰（RNAi）的效应复合物）的激活。在这里，我们报告了三个晶体结构的Nanoarchaeum equitans（Ne）C3 PO。apo-NeC 3 PO结构采用开放的形式，并首次解开了潜在的衬底入口通道。NeC 3 PO：ssRNA和NeC 3 PO：ssDNA复合物像封闭的足球一样折叠，底物被捕获在内腔。NeC 3 PO：ssRNA结构代表了迄今为止唯一可用的催化形式C3 PO复合物;通过诱变和体外切割试验，该结构提供了对真核C3 PO共享的底物结合和双阳离子辅助催化机制的重要见解。这里提出的工作进一步推进了我们对RNA干扰途径的理解。
Translin and translin-associated factor-x are highly conserved in eukaroytes; they can form heteromeric complexes (known as C3POs) and participate in various nucleic acid metabolism pathways. In humans and Drosophila, C3POs cleave the fragmented siRNA passenger strands and facilitate the activation of RNA-induced silencing complex, the effector complex of RNA interference (RNAi). Here, we report three crystal structures of Nanoarchaeum equitans (Ne) C3PO. The apo-NeC3PO structure adopts an open form and unravels a potential substrates entryway for the first time. The NeC3PO:ssRNA and NeC3PO:ssDNA complexes fold like closed football with the substrates captured at the inner cavities. The NeC3PO:ssRNA structure represents the only catalytic form C3PO complex available to date; with mutagenesis and in vitro cleavage assays, the structure provides critical insights into the substrate binding and the two-cation-assisted catalytic mechanisms that are shared by eukaryotic C3POs. The work presented here further advances our understanding on the RNAi pathway.
登录
查看更多内容
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
64.5
作者：
Gan, JH;Tropea, JE;Ji, XH
通讯作者：
Ji, XH
影响因子：
64.8
作者：
Lu, R;Maduro, M;Ding, SW
通讯作者：
Ding, SW
影响因子：
3.5
作者：
Gupta, GD;Makde, RD;Rao, BJ
通讯作者：
Rao, BJ
影响因子：
64.5
作者：
Elkayam E;Kuhn CD;Tocilj A;Haase AD;Greene EM;Hannon GJ;Joshua-Tor L
通讯作者：
Joshua-Tor L