Identification of candidate susceptibility and resistance genes of mice infected with Streptococcus suis type 2.
Identification of candidate susceptibility and resistance genes of mice infected with Streptococcus suis type 2.
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2型猪链球菌感染小鼠候选易感基因和耐药基因的鉴定
DOI：
10.1371/journal.pone.0032150
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yao H
中科院分区：
文献类型：
--
作者：
Rong J;Zhang W;Wang X;Fan H;Lu C;Yao H
猪链球菌2型（Streptococcus suis type 2，SS 2）是一种重要的猪源性致病菌和人畜共患病。A/J小鼠比C57 BL/6（B6）小鼠更容易感染SS 2，但遗传基础在很大程度上是未知的。在此，使用Illumina小鼠BeadChips鉴定了SS 2（菌株HA 9801）感染小鼠中基因表达的改变。微阵列分析显示，由于SS 2感染，A/J和B6小鼠之间的腹腔巨噬细胞中差异表达的基因有3，692个。在SS 2感染的A/J小鼠和对照A/J小鼠之间，2646个基因差异表达（1469个上调; 1177个下调）。在SS 2感染的B6和对照B6小鼠之间，1449个基因差异表达（778个上调; 671个下调）。使用京都基因和基因组百科全书（KEGG）数据库分析这些基因的重要基因本体（GO）类别和信号传导途径以产生信号传导网络。在A/J和B6小鼠中上调的基因与对细菌的应答、免疫应答、B细胞受体信号通路的正调控、I型干扰素的生物合成、防御和炎症反应有关。另外，SS 2感染B6小鼠中上调的基因涉及抗原加工和外源肽提呈、肽抗原稳定、淋巴细胞分化调节、单核细胞分化的正向调节、抗原受体介导的信号通路和吞噬功能的正向调节。在SS 2感染的B6小鼠中下调的基因在糖酵解、碳水化合物代谢过程、氨基酸代谢、行为和肌肉调节中发挥作用。通过14个代表性失调基因的定量实时PCR（qRT-PCR）验证微阵列结果。在SS 2感染的A/J和B6小鼠之间差异表达的四个基因，toll样受体2（Tlr 2）、肿瘤坏死因子（Tnf）、基质金属蛋白酶9（Mmp 9）和五聚蛋白3（Ptx 3），先前被认为与对S.猪感染。本研究确定了可能影响A/J和B6小鼠对SS 2感染的易感性或抗性的候选基因，为这些模型提供了进一步的验证，并有助于理解S。猪的致病机制。
Streptococcus suis type 2 (SS2) is an important swine pathogen and zoonosis agent. A/J mice are significantly more susceptible than C57BL/6 (B6) mice to SS2 infection, but the genetic basis is largely unknown. Here, alterations in gene expression in SS2 (strain HA9801)-infected mice were identified using Illumina mouse BeadChips. Microarray analysis revealed 3,692 genes differentially expressed in peritoneal macrophages between A/J and B6 mice due to SS2 infection. Between SS2-infected A/J and control A/J mice, 2646 genes were differentially expressed (1469 upregulated; 1177 downregulated). Between SS2-infected B6 and control B6 mice, 1449 genes were differentially expressed (778 upregulated; 671 downregulated). These genes were analyzed for significant Gene Ontology (GO) categories and signaling pathways using the Kyoto Encylopedia of Genes and Genomes (KEGG) database to generate a signaling network. Upregulated genes in A/J and B6 mice were related to response to bacteria, immune response, positive regulation of B cell receptor signaling pathway, type I interferon biosynthesis, defense and inflammatory responses. Additionally, upregulated genes in SS2-infected B6 mice were involved in antigen processing and presentation of exogenous peptides, peptide antigen stabilization, lymphocyte differentiation regulation, positive regulation of monocyte differentiation, antigen receptor-mediated signaling pathway and positive regulation of phagocytosis. Downregulated genes in SS2-infected B6 mice played roles in glycolysis, carbohydrate metabolic process, amino acid metabolism, behavior and muscle regulation. Microarray results were verified by quantitative real-time PCR (qRT-PCR) of 14 representative deregulated genes. Four genes differentially expressed between SS2-infected A/J and B6 mice, toll-like receptor 2 (Tlr2), tumor necrosis factor (Tnf), matrix metalloproteinase 9 (Mmp9) and pentraxin 3 (Ptx3), were previously implicated in the response to S. suis infection. This study identified candidate genes that may influence susceptibility or resistance to SS2 infection in A/J and B6 mice, providing further validation of these models and contributing to understanding of S. suis pathogenic mechanisms.
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影响因子：
3.1
作者：
Leib, SL;Leppert, D;Täuber, MG
通讯作者：
Täuber, MG
影响因子：
4.6
作者：
Lemay, AM;Haston, CK
通讯作者：
Haston, CK
影响因子：
3.3
作者：
Gottschalk, M;Segura, M
通讯作者：
Segura, M
影响因子：
11.2
作者：
Chemnitz, JM;Driesen, J;Schultze, JL
通讯作者：
Schultze, JL
影响因子：
3.3
作者：
Kolb, SA;Lahrtz, F;Fontana, A
通讯作者：
Fontana, A