Silver-Gold Nanocomposite Substrates for Metal-Enhanced Fluorescence: Ensemble and Single-Molecule Spectroscopic Studies.
Silver-Gold Nanocomposite Substrates for Metal-Enhanced Fluorescence: Ensemble and Single-Molecule Spectroscopic Studies.
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DOI：
10.1021/jp212242x
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发表时间：
2012-03-01
影响因子：
3.7
通讯作者：
Lakowicz, Joseph R.
中科院分区：
文献类型：
--
作者：
Choudhury, Sharmistha Dutta;Badugu, Ramachandram;Ray, Krishanu;Lakowicz, Joseph R.
近年来，荧光团与等离子体纳米结构或纳米颗粒相互作用的研究引起了人们越来越大的兴趣。这些相互作用导致几个有利的效果，如荧光强度的增加，增加的光稳定性，并减少激发态寿命，可以利用，以提高目前的荧光方法的能力。在这方面，我们报告使用新开发的银-金纳米复合材料（银-金-NC）的结构作为基板的金属增强荧光（MEF）。通过一步电置换反应在载玻片上制备了薄的银膜，制备了Ag-Au-NC基片。该方法简单，适用于大面积MEF基片的制备。我们已经观察到约15倍的增强ATTO 655的荧光强度从合奏荧光测量使用这些基板。在Ag-Au-NC基底上的荧光增强还伴随着ATTO 655的荧光寿命的降低，这与荧光团-等离子体激元耦合机制一致。单分子荧光测量已被执行，以获得更多的洞察到金属荧光团的相互作用，并解开的异质性与制造基板的个别荧光团的相互作用。单分子的研究是在很好的协议与合奏测量，并显示最大的增强~50倍的分子位于附近的基板上的“热点”。从本质上讲，Ag-Au-NC基板具有非常好的潜力，用于各种MEF应用。
In recent years, there has been a growing interest in the studies involving the interactions of fluorophores with plasmonic nanostructures or nanoparticles. These interactions lead to several favorable effects such as increase in the fluorescence intensities, increased photostabilities, and reduced excited-state lifetimes that can be exploited to improve the capabilities of present fluorescence methodologies. In this regard, we report the use of newly developed silver–gold nanocomposite (Ag–Au–NC) structures as substrates for metal-enhanced fluorescence (MEF). The Ag–Au–NC substrates have been prepared by a one-step galvanic replacement reaction from thin silver films coated on glass slides. This approach is simple and suitable for the fabrication of MEF substrates with large area. We have observed about 15-fold enhancement in the fluorescence intensity of ATTO655 from ensemble fluorescence measurements using these substrates. The fluorescence enhancement on the Ag–Au–NC substrates is also accompanied by a reduction in the fluorescence lifetime of ATTO655, which is consistent with the fluorophore–plasmon coupling mechanism. Single-molecule fluorescence measurements have been performed to gain more insight into the metal–fluorophore interactions and to unravel the heterogeneity in the interaction of individual fluorophores with the fabricated substrates. The single-molecule studies are in good agreement with the ensemble measurements and show maximum enhancements of ~50-fold for molecules located in proximity to the “hotspots” on the substrates. In essence, the Ag–Au–NC substrates have a very good potential for various MEF applications.
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影响因子：
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通讯作者：
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