Longitudinal tracking of human dendritic cells in murine models using magnetic resonance imaging.
Longitudinal tracking of human dendritic cells in murine models using magnetic resonance imaging.
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DOI：
10.1002/mrm.22519
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发表时间：
2010-11
影响因子：
3.3
通讯作者：
Merad, Miriam
中科院分区：
文献类型：
--
作者：
Briley-Saebo, Karen C.;Leboeuf, Marylene;Dickson, Stephen;Mani, Venkatesh;Fayad, Zahi A.;Palucka, A. Karolina;Banchereau, Jacques;Merad, Miriam
离体产生的树突状细胞目前用于在实体瘤中诱导治疗性免疫。有效的免疫应答需要树突状细胞回家并留在淋巴器官中，以允许与T淋巴细胞充分相互作用。本研究的目的是使用磁共振成像检测和跟踪小鼠模型中菲立磁标记的人树突状细胞。将人树突状细胞与菲立磁共同孵育，评价标记对树突状细胞免疫功能的影响。使用离体树突状细胞模型来评估磁共振方法的灵敏度，并在静脉内或皮下注射后评估体内归巢。获得肝脏、脾脏和引流淋巴结的R2* 图，并使用电感耦合等离子体质谱法或弛豫法定量菲立磁组织浓度。然后确定体内R2* 值和铁含量之间的相关性。菲立磁标记不影响树突状细胞的成熟或功能。体模结果表明，在给定切片内可以检测到125个树突状细胞。观察到体内R2* 值与铁沉积之间的强相关性。重要的是，在静脉注射后2周内，在脾脏中检测到菲立磁标记的树突状细胞。这项研究表明，磁共振成像可用于纵向跟踪菲立磁标记的人树突状细胞长达2周后注射。
Ex vivo generated dendritic cells are currently used to induce therapeutic immunity in solid tumors. Effective immune response requires dendritic cells to home and remain in lymphoid organs to allow for adequate interaction with T lymphocytes. The aim of the current study was to detect and track Feridex labeled human dendritic cells in murine models using magnetic resonance imaging. Human dendritic cells were incubated with Feridex and the effect of labeling on dendritic cells immune function was evaluated. Ex vivo dendritic cell phantoms were used to estimate sensitivity of the magnetic resonance methods and in vivo homing was evaluated after intravenous or subcutaneous injection. R2*-maps of liver, spleen, and draining lymph nodes were obtained and inductively coupled plasma mass spectrometry or relaxometry methods were used to quantify the Feridex tissue concentrations. Correlations between in vivo R2* values and iron content were then determined. Feridex labeling did not affect dendritic cell maturation or function. Phantom results indicated that it was possible to detect 125 dendritic cells within a given slice. Strong correlation between in vivo R2* values and iron deposition was observed. Importantly, Feridex-labeled dendritic cells were detected in the spleen for up to 2 weeks postintravenous injection. This study suggests that magnetic resonance imaging may be used to longitudinally track Feridex-labeled human dendritic cells for up to 2 weeks after injection.
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影响因子：
3.3
作者：
Kim, Daehong;Chun, Byoung-Gi;Song, Jihwan
通讯作者：
Song, Jihwan
使用MRI研究横向松弛率，扩散系数和标记的细胞浓度之间的关系。
影响因子：
3.3
作者：
Athiraman, Hernanthkumar;Jiang, Quan;Ding, Guang Liang;Zhang, Li;Zhang, Zheng Gang;Wang, Lei;Arbab, Ali S.;Li, Qingjiang;Panda, Swayam;Ledbetter, Karen;Rad, Ali M.;Chopp, Michael
通讯作者：
Chopp, Michael
DOI：
10.2967/jnumed.108.053546
发表时间：
2009-02
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
Budde MD;Frank JA
通讯作者：
Frank JA
影响因子：
5.2
作者：
Maxwell, Dustin J.;Bonde, Jesper;Nolta, Jan A.
通讯作者：
Nolta, Jan A.
影响因子：
3.3
作者：
Liu, Wei;Frank, Joseph A.
通讯作者：
Frank, Joseph A.