Processing sites in the human immunodeficiency virus type 1 (HIV-1) Gag-Pro-Pol precursor are cleaved by the viral protease at different rates.
Processing sites in the human immunodeficiency virus type 1 (HIV-1) Gag-Pro-Pol precursor are cleaved by the viral protease at different rates.
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DOI：
10.1186/1742-4690-2-66
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发表时间：
2005-11-01
期刊：
影响因子：
3.3
通讯作者：
Swanstrom R
中科院分区：
文献类型：
--
作者：
Pettit SC;Lindquist JN;Kaplan AH;Swanstrom R
我们研究了HIV-1 Gag-Pro-Pol前体在体外试验中的加工动力学，该试验中加入了反式成熟蛋白酶。加工位点以不同的速率裂解以产生不同的中间体。初始裂解发生在p2/NC位点。中间裂解发生在MA/CA和RT/IN网站，并在较小程度上在RT上游的网站。晚裂解发生在侧翼的蛋白酶（PR）结构域的网站，这表明这些网站的隔离。我们观察到成对的中间体，表明RT/RH位点的半切割，表明在这些测定条件下，Gag-Pro-Pol中的RT结构域呈二聚体形式。这些结果阐明了我们对Gag-Pro-Pol前体的加工动力学的理解，并表明受调节的裂解。我们的研究结果进一步表明，PR和RT结构域的早期二聚化可以作为一个调节元件，影响Pol结构域内的加工动力学。
We have examined the kinetics of processing of the HIV-1 Gag-Pro-Pol precursor in an in vitro assay with mature protease added in trans. The processing sites were cleaved at different rates to produce distinct intermediates. The initial cleavage occurred at the p2/NC site. Intermediate cleavages occurred at similar rates at the MA/CA and RT/IN sites, and to a lesser extent at sites upstream of RT. Late cleavages occurred at the sites flanking the protease (PR) domain, suggesting sequestering of these sites. We observed paired intermediates indicative of half- cleavage of RT/RH site, suggesting that the RT domain in Gag-Pro-Pol was in a dimeric form under these assay conditions. These results clarify our understanding of the processing kinetics of the Gag-Pro-Pol precursor and suggest regulated cleavage. Our results further suggest that early dimerization of the PR and RT domains may serve as a regulatory element to influence the kinetics of processing within the Pol domain.
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影响因子：
2.9
作者：
Louis, JM;Dyda, F;Davies, DR
通讯作者：
Davies, DR
影响因子：
5.4
作者：
Halwani, R;Khorchid, A;Kleiman, L
通讯作者：
Kleiman, L
DOI：
10.1073/pnas.86.3.807
发表时间：
1989-02-01
影响因子：
11.1
作者：
KRAUSSLICH, HG;INGRAHAM, RH;CARTER, CA
通讯作者：
CARTER, CA
影响因子：
3.7
作者：
KARACOSTAS, V;WOLFFE, EJ;MOSS, B
通讯作者：
MOSS, B
影响因子：
5.4
作者：
CRAVEN, RC;BENNETT, RP;WILLS, JW
通讯作者：
WILLS, JW