Calpain regulates actin remodeling during cell spreading.
Calpain regulates actin remodeling during cell spreading.
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DOI：
10.1083/jcb.141.3.647
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发表时间：
1998-05-04
期刊：
影响因子：
--
通讯作者：
Herman IM
中科院分区：
文献类型：
--
作者：
Potter DA;Tirnauer JS;Janssen R;Croall DE;Hughes CN;Fiacco KA;Mier JW;Maki M;Herman IM
先前的研究表明，钙依赖性蛋白酶，钙蛋白酶，参与创伤愈合过程中的肌动蛋白细胞骨架的重塑，并在细胞迁移过程中是活跃的。为了直接测试钙蛋白酶在细胞铺展中发挥的作用，分离了几种NIH-3 T3衍生的克隆细胞系，其过表达钙蛋白酶的生物抑制剂钙蛋白酶抑制剂。这些细胞稳定过表达钙蛋白酶抑制蛋白2至8倍，相对于控制和不同的亲本和对照细胞系的形态，扩散，细胞骨架结构，和生化特性。突变细胞的形态学特征包括不能延伸板状伪足，以及异常的丝状伪足、延伸和收缩。而野生型细胞在平板接种后30分钟内延伸lamellum，所有的钙蛋白酶抑制蛋白过表达细胞系未能传播和组装肌动蛋白丰富的过程。经遗传改变以过表达钙蛋白酶抑制素的细胞显示原位或体外测量的钙蛋白酶活性降低。由于钙蛋白酶抑制，ERM蛋白ezrin而不是radixin或膜突蛋白显著增加。为了证实钙蛋白酶活性的抑制与扩散缺陷有关，还分析了钙蛋白酶的药理学抑制剂。细胞渗透抑制剂钙肽酶和MDL 28，170引起扩散的立即抑制。丝状伪足形成和板层延伸密切相关的过程的失败表明钙蛋白酶密切参与肌动蛋白重塑和细胞铺展。
Previous studies suggest that the Ca2+-dependent proteases, calpains, participate in remodeling of the actin cytoskeleton during wound healing and are active during cell migration. To directly test the role that calpains play in cell spreading, several NIH-3T3– derived clonal cell lines were isolated that overexpress the biological inhibitor of calpains, calpastatin. These cells stably overexpress calpastatin two- to eightfold relative to controls and differ from both parental and control cell lines in morphology, spreading, cytoskeletal structure, and biochemical characteristics. Morphologic characteristics of the mutant cells include failure to extend lamellipodia, as well as abnormal filopodia, extensions, and retractions. Whereas wild-type cells extend lamellae within 30 min after plating, all of the calpastatin-overexpressing cell lines fail to spread and assemble actin-rich processes. The cells genetically altered to overexpress calpastatin display decreased calpain activity as measured in situ or in vitro. The ERM protein ezrin, but not radixin or moesin, is markedly increased due to calpain inhibition. To confirm that inhibition of calpain activity is related to the defect in spreading, pharmacological inhibitors of calpain were also analyzed. The cell permeant inhibitors calpeptin and MDL 28, 170 cause immediate inhibition of spreading. Failure of the intimately related processes of filopodia formation and lamellar extension indicate that calpain is intimately involved in actin remodeling and cell spreading.
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影响因子：
2.9
作者：
CROALL, DE;MCGRODY, KS
通讯作者：
MCGRODY, KS
影响因子：
4.1
作者：
ANAGLI, J;HAGMANN, J;SHAW, E
通讯作者：
SHAW, E
影响因子：
64.5
作者：
BECKERLE, MC;BURRIDGE, K;CROALL, DE
通讯作者：
CROALL, DE
影响因子：
64.8
作者：
HAHN, K;DEBIASIO, R;TAYLOR, DL
通讯作者：
TAYLOR, DL
DOI：
10.1083/jcb.120.1.129
发表时间：
1993-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Algrain M;Turunen O;Vaheri A;Louvard D;Arpin M
通讯作者：
Arpin M