Comparative de novo transcriptomics and untargeted metabolomic analyses elucidate complicated mechanisms regulating celery (Apium graveolens L.) responses to selenium stimuli
Comparative de novo transcriptomics and untargeted metabolomic analyses elucidate complicated mechanisms regulating celery (Apium graveolens L.) responses to selenium stimuli
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比较从头转录组学和非靶向代谢组学分析阐明了调节芹菜（Apiumgravolelens L.）对硒刺激反应的复杂机制
DOI：
10.1371/journal.pone.0226752
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发表时间：
2019-12
期刊：
影响因子：
3.7
通讯作者：
Chenliang Yu
中科院分区：
文献类型：
--
作者：
Chenghao Zhang;Baoyu Xu;Cheng-Ri Zhao;Junwei Sun;Qixian Lai;Chenliang Yu
目前，人们越来越关注硒对环境和生物体的影响。土壤中过多的硒对植物有害。在这项研究中，Illumina的RNA测序和非靶向代谢物组的控制和硒处理的芹菜幼苗进行了分析。总共获得了297，911，046个干净的读段，并组装成150，218个转录物（50，876个单基因）。使用不同的数据库共注释了36，287个unigenes。此外，8,907差异表达的基因，包括5,319和3,588下调基因，被确定之间的模拟和硒处理的植物。“苯丙素类生物合成”是KEGG最丰富的途径。共有24个硫和硒化合物代谢单基因差异表达。此外，使用非靶向代谢组学方法鉴定了1，774种代谢物和237种显著差异累积的代谢物。我们对差异表达基因和累积代谢产物的富集KEGG途径进行了相关性分析。研究结果表明，芹菜硒耐性的调控可能与芹菜中的候选基因和代谢产物有关。这些结果加深了我们对芹菜适应硒胁迫的分子机制的理解。
Presently, concern regarding the effects of selenium (Se) on the environment and organisms worldwide is increasing. Too much Se in the soil is harmful to plants. In this study, Illumina RNA sequencing and the untargeted metabolome of control and Se-treated celery seedlings were analyzed. In total, 297,911,046 clean reads were obtained and assembled into 150,218 transcripts (50,876 unigenes). A total of 36,287 unigenes were annotated using different databases. Additionally, 8,907 differentially expressed genes, including 5,319 up- and 3,588 downregulated genes, were identified between mock and Se-treated plants. “Phenylpropanoid biosynthesis” was the most enriched KEGG pathway. A total of 24 sulfur and selenocompound metabolic unigenes were differentially expressed. Furthermore, 1,774 metabolites and 237 significant differentially accumulated metabolites were identified using the untargeted metabolomic approach. We conducted correlation analyses of enriched KEGG pathways of differentially expressed genes and accumulated metabolites. Our findings suggested that candidate genes and metabolites involved in important biological pathways may regulate Se tolerance in celery. The results increase our understanding of the molecular mechanism responsible for celery’s adaptation to Se stress.
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DOI：
10.2307/2401526
发表时间：
1969-12
期刊：
--
影响因子：
--
作者：
Van der Plank
通讯作者：
Van der Plank
影响因子：
13.8
作者：
Cakir, Ozgur;Candar-Cakir, Bilgin;Zhang, Baohong
通讯作者：
Zhang, Baohong
影响因子：
4.9
作者：
Chengyi Zhao;Jinghua Ren;C. Xue;E. Lin
通讯作者：
Chengyi Zhao;Jinghua Ren;C. Xue;E. Lin
DOI：
10.1111/tpj.12544
发表时间：
2014-07
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Yanxia Xu;Saina Zhang;Haipeng Guo;Suikang Wang;Ligen Xu;Chuanyou Li;Q. Qian;Fan Chen;Markus Geisler;Yanhua Qi;D. Jiang
通讯作者：
Yanxia Xu;Saina Zhang;Haipeng Guo;Suikang Wang;Ligen Xu;Chuanyou Li;Q. Qian;Fan Chen;Markus Geisler;Yanhua Qi;D. Jiang
影响因子：
2.9
作者：
Yi-hua Zhan;Cheng-hao Zhang;Qiu-xun Zheng;Zong-an Huang;Chen Yu
通讯作者：
Yi-hua Zhan;Cheng-hao Zhang;Qiu-xun Zheng;Zong-an Huang;Chen Yu