In vitro real-time magnetic resonance imaging for quantification of thrombosis.
In vitro real-time magnetic resonance imaging for quantification of thrombosis.
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DOI：
10.1007/s10334-020-00872-2
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Manning KB
中科院分区：
文献类型：
--
作者：
Yang L;Neuberger T;Manning KB
血栓形成是心血管装置失效的主要原因。虽然计算模拟是预测血栓形成和评估医疗设备风险的强大工具，但可用于验证模拟的实验数据有限。本研究的目的是为装置诱发血栓提供生长血栓的实验数据。采用牛和人的血液在BFS模型中循环30 min，流速恒定为0.76 L/min，研究了BFS内的血栓形成或突然扩张。实时三维流补偿磁共振成像（MRI），辅以Magnevist（一种改善血栓描绘的造影剂），用于量化模型内血栓沉积和生长。研究表明，BFS模型诱导了血流再循环区，促进了血栓形成。30min时，人血血栓形成的长度（13.3±0.6 vs. 18.1±1.3 mm）、高度（2.3±0.1 vs. 2.6±0.04 mm）、暴露于血液的表面积（0.67±0.03 vs. 1.05±0.08 cm2）和体积（0.069±0.004 vs. 0.093±0.007 cm3）均小于牛血，p < 0.01。排除血流再循环影响的血栓测量归一化表明，血栓大小在前15分钟增加，20分钟后稳定。血液特性，包括粘度、红细胞压积和血小板计数影响血栓形成。首次使用造影剂支持的实时MRI来研究突然扩张中的血栓沉积和生长。本研究为器械诱发血栓形成提供了实验数据，对血栓形成计算模拟的验证具有重要价值。
Thrombosis is a leading cause of failure for cardiovascular devices. While computational simulations are a powerful tool to predict thrombosis and evaluate the risk for medical devices, limited experimental data are available to validate the simulations. The aim of the current study is to provide experimental data of a growing thrombus for device-induced thrombosis. Thrombosis within a backward-facing step (BFS), or sudden expansion was investigated, using bovine and human blood circulated through the BFS model for 30 min, with a constant inflow rate of 0.76 L/min. Real-time three-dimensional flow compensated magnetic resonance imaging (MRI), supported with Magnevist, a contrast agent improving thrombus delineation, was applied to quantify thrombus deposition and growth within the model. The study showed that the BFS model induced a flow recirculation region, which facilitated thrombosis. By 30 min, in comparison to bovine blood, human blood resulted in smaller thrombus formation, in terms of the length (13.3±0.6 vs. 18.1±1.3 mm), height (2.3±0.1 vs. 2.6±0.04 mm), surface area exposed to blood (0.67±0.03 vs 1.05±0.08 cm2), and volume (0.069±0.004 vs. 0.093±0.007 cm3), with p < 0.01. Normalization of the thrombus measurements, which excluded the flow recirculation effects, suggested that the thrombus sizes increased during the first 15 min and stabilized after 20 min. Blood properties, including viscosity, hematocrit, and platelet count affected thrombosis. For the first time, contrast agent supported real-time MRI was performed to investigate thrombus deposition and growth within a sudden expansion. This study provides experimental data for device-induced thrombosis, which is valuable for validation of computational thrombosis simulations.
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DOI：
10.1016/j.jtcvs.2003.12.013
发表时间：
2004-05-01
影响因子：
6
作者：
Dürrleman, N;Pellerin, M;Carrier, M
通讯作者：
Carrier, M
影响因子：
3.5
作者：
Rojano, Rodrigo Mendez;Mendez, Simon;Nicoud, Franck
通讯作者：
Nicoud, Franck
影响因子：
8.9
作者：
Kirklin, James K.;Naftel, David C.;Miller, Marissa A.
通讯作者：
Miller, Marissa A.
影响因子：
4.4
作者：
Goyen, M;Lauenstein, TC;Ruehm, SG
通讯作者：
Ruehm, SG
影响因子：
5
作者：
Kluge, A;Mueller, C;Bachmann, G
通讯作者：
Bachmann, G