Rate and mechanism of nonhomologous recombination during a single cycle of retroviral replication.
Rate and mechanism of nonhomologous recombination during a single cycle of retroviral replication.
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逆转录病毒复制单个周期期间非同源重组的速率和机制。
DOI：
10.1126/science.8421784
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Temin,HM
中科院分区：
文献类型：
--
作者：
Zhang,J;Temin,HM
在Rous肉瘤病毒等逆转录病毒中发现的癌基因是通过将细胞原癌基因转导到病毒基因组中而产生的。在不同的病毒中发现了几种不同类型的病毒和原癌基因序列之间的连接。开发了一种逆转录病毒载体系统和一种在逆转录病毒复制的单个周期内模拟这种转导的方案。转导包括病毒-细胞嵌合RNA的形成，逆转录生长点从感染性逆转录病毒到嵌合RNA的链切换，以及在病毒DNA合成的其余部分通常随后的缺失。序列同一性的短区域经常用于链切换。这一过程的速率约为同源逆转录病毒重组速率的0.1%至1%。
Oncogenes discovered in retroviruses such as Rous sarcoma virus were generated by transduction of cellular proto-oncogenes into the viral genome. Several different kinds of junctions between the viral and proto-oncogene sequences have been found in different viruses. A system of retrovirus vectors and a protocol that mimicked this transduction during a single cycle of retrovirus replication was developed. The transduction involved the formation of a chimeric viral-cellular RNA, strand switching of the reverse transcription growing point from an infectious retrovirus to the chimeric RNA, and often a subsequent deletion during the rest of viral DNA synthesis. A short region of sequence identity was frequently used for the strand switching. The rate of this process was about 0.1 to 1 percent of the rate of homologous retroviral recombination.
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影响因子：
5.6
作者：
HUANG, CC;HAMMOND, C;BISHOP, JM
通讯作者：
BISHOP, JM
DOI：
10.1073/pnas.87.4.1556
发表时间：
1990-02-01
影响因子：
11.1
作者：
HU, WS;TEMIN, HM
通讯作者：
TEMIN, HM
有证据表明逆转录病毒转导是由 DNA 而不是 RNA 介导的。
DOI：
10.1073/pnas.87.9.3604
发表时间：
1990
影响因子：
11.1
作者：
Goodrich,DW;Duesberg,PH
通讯作者：
Duesberg,PH
影响因子：
56.9
作者：
SWAIN, A;COFFIN, JM
通讯作者：
COFFIN, JM
影响因子：
64.5
作者：
JUNGHANS, RP;BOONE, LR;SKALKA, AM
通讯作者：
SKALKA, AM