Involvement of the Wbp pathway in the biosynthesis of Porphyromonas gingivalis lipopolysaccharide with anionic polysaccharide.
Involvement of the Wbp pathway in the biosynthesis of Porphyromonas gingivalis lipopolysaccharide with anionic polysaccharide.
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DOI：
10.1038/srep05056
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发表时间：
2014-05-23
影响因子：
4.6
通讯作者：
Nakayama K
中科院分区：
文献类型：
--
作者：
Shoji M;Sato K;Yukitake H;Naito M;Nakayama K
牙周病原菌牙龈卟啉单胞菌有两种不同的脂多糖分子，即O-内毒素和A-内毒素。我们最近发现牙龈假单胞菌HG66株缺乏A-内毒素。在这里，我们发现，将野生型wbpB基因导入菌株HG66，恢复了A-内毒素的形成。对HG66菌株的wbpB基因进行测序，发现该基因存在无义突变。WbpB基因产物是WBP途径的一员，在铜绿假单胞菌UDP-ManNAc(3NAc)A的合成中起作用；UDP-ManNAc(3NAc)A是由WbpA、WbpB、WbpE、WbpD和WbpI蛋白依次合成的。然后，我们测定了pGN_0002基因(wbpD同源基因)对A-LPS生物合成的影响。PGN_0002缺失突变体表现出A-内毒素生物合成缺陷。综合前人的研究，本研究结果表明，WBP途径合成的最终产物是A-内毒素生物合成所必需的糖底物之一。
The periodontal pathogen Porphyromonas gingivalis has two different lipopolysaccharide (LPS) molecules, O-LPS and A-LPS. We have recently shown that P. gingivalis strain HG66 lacks A-LPS. Here, we found that introduction of a wild-type wbpB gene into strain HG66 restored formation of A-LPS. Sequencing of the wbpB gene from strain HG66 revealed the presence of a nonsense mutation in the gene. The wbpB gene product is a member of the Wbp pathway, which plays a role in the synthesis of UDP-ManNAc(3NAc)A in Pseudomonas aeruginosa; UDP-ManNAc(3NAc)A is sequentially synthesized by the WbpA, WbpB, WbpE, WbpD and WbpI proteins. We then determined the effect of the PGN_0002 gene, a wbpD homolog, on the biosynthesis of A-LPS. A PGN_0002-deficient mutant demonstrated an A-LPS biosynthesis deficiency. Taken together with previous studies, the present results suggest that the final product synthesized by the Wbp pathway is one of the sugar substrates necessary for the biosynthesis of A-LPS.
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影响因子：
3.1
作者：
Kelly, John;Logan, Susan M.;Vinogradov, Evgeny
通讯作者：
Vinogradov, Evgeny
影响因子：
3.1
作者：
Aruni, A. Wilson;Lee, J.;Fletcher, H. M.
通讯作者：
Fletcher, H. M.
影响因子：
3.1
作者：
O'Brien-Simpson, NM;Paolini, RA;Reynolds, EC
通讯作者：
Reynolds, EC
DOI：
10.1046/j.1432-1033.2003.03644.x
发表时间：
2003-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Hashimoto, M;Asai, Y;Ogawa, T
通讯作者：
Ogawa, T
影响因子：
3.1
作者：
Kondo, Yoshio;Ohara, Naoya;Nakayama, Koji
通讯作者：
Nakayama, Koji