Effect of phloretin on growth performance, serum biochemical parameters and antioxidant profile in heat-stressed broilers.
Effect of phloretin on growth performance, serum biochemical parameters and antioxidant profile in heat-stressed broilers.
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DOI：
10.1016/j.psj.2021.101217
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发表时间：
2021-08
期刊：
影响因子：
4.4
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Hu H;Bai X;Xu K;Zhang C;Chen L
本研究旨在探讨根皮素对热应激肉鸡生长性能、血清生化指标、抗氧化能力、谷胱甘肽（GSH）相关酶、红细胞核因子2（Nrf 2）和热休克蛋白70（HSP 70）的影响。选用22日龄爱拔益加肉鸡240只，随机分为4组。对照组饲养于23.0 ± 0.61°C，饲喂基础日粮;热应激组（A、B和C组）饲养于30.5 ± 0.69°C，饲喂基础日粮，基础日粮中分别添加根皮素0、100和200 mg/kg。血清取自42日龄肉仔鸡。结果表明，热应激降低（P < 0.05）体重、增重、采食量、血清总蛋白（TP）、甘油三酯（TG）、三碘甲状腺原氨酸（T3）、甲状腺素（T4）、谷胱甘肽（GSH）、过氧化氢酶（CAT）和总抗氧化能力（T-AOC）水平;但与对照组相比，饲料增重比（FGR）和血清丙二醛（MDA）水平显著升高（P < 0.05）。在热应激组中，添加200 mg/kg根皮素可显著提高肉仔鸡的FBW、BWG、FI、血清TP、TG、T4、GSH、CAT和T-AOC水平（P <0.05），降低FGR和血清MDA水平（P <0.05）。热应激处理后，肉鸡血清谷胱甘肽过氧化物酶（GSH-Px）、γ-谷氨酰半胱氨酸合成酶（γ-GCS）和Nrf 2含量显著降低（P <0.05），而HSP 70含量显著升高（P <0.05）。在热应激组中，添加200 mg/kg根皮素显著提高（P < 0.05）热应激肉鸡血清GSH-Px、γ-GCS和Nrf 2水平，显著降低（P < 0.05）热应激肉鸡血清HSP 70水平。在高温条件下，根皮素包合物对FBW、BWG、FI、FGR、血清TP、TG、T4、MDA、GSH、CAT、T-AOC、GSH-Px、γ-GCS、Nrf 2和HSP 70的影响呈线性关系。这些结果表明，根皮素可以通过调节GSH相关酶Nrf 2和HSP 70来改善热应激肉鸡的生长性能、血清指标和抗氧化能力。
The objective of this work was to evaluate the effect of phloretin on growth performance, serum biochemical parameters, antioxidant profile, glutathione (GSH)-related enzymes, nuclear factor erythroid 2–related 2 (Nrf2) and heat shock protein 70 (HSP70) in heat-stressed broilers. A total of 240, 22-day-old Arbor Acres broilers were divided into 4 groups. The control group was housed at 23.0 ± 0.61°C and fed with basal diet, while the 3 heat-stressed groups (A, B, and C groups) were housed at 30.5 ± 0.69°C and fed with basal diet containing 0, 100, and 200 mg/kg phloretin, respectively. Serum was taken form 42-day-old broilers. Results showed that heat stress decreased (P < 0.05) the final body weight (FBW), body weight gain (BWG), feed intake (FI), serum total protein (TP), triglyceride (TG), triiodothyronine (T3), thyroxine (T4), GSH, catalase (CAT), and total antioxidant capacity (T-AOC) levels, but increased (P < 0.05) the feed-to-gain ratio (FGR) and serum malondialdehyde (MDA) levels in broilers compared with that in the control group. Among the heat-stressed groups, supplementary 200 mg/kg phloretin increased (P < 0.05) the FBW, BWG, FI, serum TP, TG, T4, GSH, CAT, and T-AOC levels, and decreased (P < 0.05) the FGR and serum MDA in broilers. There were significant decreases (P < 0.05) in the glutathione peroxidase (GSH-Px), γ-glutamylcysteine synthetase (γ-GCS), and Nrf2, but significant increases (P < 0.05) in the HSP70 of the broiler serum after heat stress treatment. Among the heat-stressed groups, supplementary 200 mg/kg phloretin increased (P < 0.05) the GSH-Px, γ-GCS, and Nrf2 levels, but decreased (P < 0.05) the serum HSP70 level in the heat-stressed broilers. Under high temperature condition, FBW, BWG, FI, FGR, serum TP, TG, T4, MDA, GSH, CAT, T-AOC, GSH-Px, γ-GCS, Nrf2 and HSP70 were linearly affected by inclusion of phloretin. These results indicated that phloretin may improve growth performance, serum parameters, and antioxidant profiles through regulated GSH-related enzymes, Nrf2 and HSP70 in heat-stressed broilers.
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