Inhibition of enveloped viruses infectivity by curcumin.
Inhibition of enveloped viruses infectivity by curcumin.
复制标题
DOI：
10.1371/journal.pone.0062482
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hsu WL
中科院分区：
文献类型：
--
作者：
Chen TY;Chen DY;Wen HW;Ou JL;Chiou SS;Chen JM;Wong ML;Hsu WL
姜黄素是一种天然化合物，也是咖喱中的一种成分，具有抗氧化、抗癌和抗氧化的特性。以前，我们报道姜黄素通过抑制血凝（HA）活性来消除流感病毒的感染性。这项研究证明了姜黄素抑制包膜病毒感染性的一种新机制。在所有分析的包膜病毒，包括流感病毒，姜黄素抑制空斑形成。相反，无包膜肠道病毒71型不受姜黄素治疗的影响。我们使用模拟病毒包膜的含荧光染料（磺酰罗丹明B; SR B）的脂质体评估姜黄素对膜结构的影响。姜黄素处理诱导SRB从这些脂质体中泄漏，并且加入流感病毒减少了泄漏，表明姜黄素破坏了病毒包膜和脂质体的膜的完整性。当测试不同直径的脂质体时，我们检测到较小尺寸的脂质体比较大的脂质体更高水平的SRB泄漏。有趣的是，流感病毒（直径约100 nm）减少空斑形成所需的姜黄素浓度低于伪狂犬病病毒（约180 nm）和牛痘病毒（约335 × 200 × 200 nm）。这些数据为姜黄素的分子抗病毒机制及其作为包膜病毒抗病毒剂的潜在用途提供了见解。
Curcumin, a natural compound and ingredient in curry, has antiinflammatory, antioxidant, and anticarcinogenic properties. Previously, we reported that curcumin abrogated influenza virus infectivity by inhibiting hemagglutination (HA) activity. This study demonstrates a novel mechanism by which curcumin inhibits the infectivity of enveloped viruses. In all analyzed enveloped viruses, including the influenza virus, curcumin inhibited plaque formation. In contrast, the nonenveloped enterovirus 71 remained unaffected by curcumin treatment. We evaluated the effects of curcumin on the membrane structure using fluorescent dye (sulforhodamine B; SRB)-containing liposomes that mimic the viral envelope. Curcumin treatment induced the leakage of SRB from these liposomes and the addition of the influenza virus reduced the leakage, indicating that curcumin disrupts the integrity of the membranes of viral envelopes and of liposomes. When testing liposomes of various diameters, we detected higher levels of SRB leakage from the smaller-sized liposomes than from the larger liposomes. Interestingly, the curcumin concentration required to reduce plaque formation was lower for the influenza virus (approximately 100 nm in diameter) than for the pseudorabies virus (approximately 180 nm) and the vaccinia virus (roughly 335 × 200 × 200 nm). These data provide insights on the molecular antiviral mechanisms of curcumin and its potential use as an antiviral agent for enveloped viruses.
登录
查看更多内容
影响因子：
5.4
作者：
Si, Xiaoning;Wang, Yahong;Luo, Honglin
通讯作者：
Luo, Honglin
影响因子：
3.8
作者：
Nimmerjahn, F;Dudziak, D;Mautner, J
通讯作者：
Mautner, J
影响因子：
3.4
作者：
Mazur, Igor;Wurzer, Walter J.;Ludwig, Stephan
通讯作者：
Ludwig, Stephan
影响因子：
5.8
作者：
Anuchapreeda, S;Leechanachai, P;Limtrakul, P
通讯作者：
Limtrakul, P
影响因子：
6.1
作者：
Maheshwari, RK;Singh, AK;Srimal, RC
通讯作者：
Srimal, RC