Bone marrow-derived monocyte infusion improves hepatic fibrosis by decreasing osteopontin, TGF-β1, IL-13 and oxidative stress.
Bone marrow-derived monocyte infusion improves hepatic fibrosis by decreasing osteopontin, TGF-β1, IL-13 and oxidative stress.
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DOI：
10.3748/wjg.v23.i28.5146
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发表时间：
2017-07-28
影响因子：
4.3
通讯作者：
de Oliveira SA
中科院分区：
文献类型：
--
作者：
de Souza VCA;Pereira TA;Teixeira VW;Carvalho H;de Castro MCAB;D'assunção CG;de Barros AF;Carvalho CL;de Lorena VMB;Costa VMA;Teixeira ÁAC;Figueiredo RCBQ;de Oliveira SA
探讨骨髓来源的CD11b+CD14+单核细胞对慢性肝损伤小鼠的治疗作用。C57BL/6小鼠经口给予四氯化碳和乙醇6mo，造成慢性肝损伤。用免疫磁分离的方法分离骨髓来源的单核细胞用于治疗。采用形态计量学、生化检测、免疫组织化学和酶联免疫吸附试验等方法评价细胞移植效果。CD11b+CD14+单核细胞治疗明显减轻肝纤维化，升高肝脏谷胱甘肽水平。单核细胞治疗组除IL-13、转化生长因子-α-1、金属蛋白酶组织抑制因子-1等促纤维化因子外，肿瘤坏死因子-β、IL-6、IL-1β等促炎细胞因子水平也降低，IL-10、基质金属蛋白酶-9水平升高。CD11b+CD14+单核细胞移植后肝脏α-平滑肌肌动蛋白和骨桥蛋白的表达发生明显变化。单核细胞治疗能够通过减少氧化应激和炎症反应，增加抗纤维化因子，从而改善肝纤维化。
To evaluate the therapeutic effects of bone marrow-derived CD11b+CD14+ monocytes in a murine model of chronic liver damage. Chronic liver damage was induced in C57BL/6 mice by administration of carbon tetrachloride and ethanol for 6 mo. Bone marrow-derived monocytes isolated by immunomagnetic separation were used for therapy. The cell transplantation effects were evaluated by morphometry, biochemical assessment, immunohistochemistry and enzyme-linked immunosorbent assay. CD11b+CD14+ monocyte therapy significantly reduced liver fibrosis and increased hepatic glutathione levels. Levels of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-6 and IL-1β, in addition to pro-fibrotic factors, such as IL-13, transforming growth factor-β1 and tissue inhibitor of metalloproteinase-1 also decreased, while IL-10 and matrix metalloproteinase-9 increased in the monocyte-treated group. CD11b+CD14+ monocyte transplantation caused significant changes in the hepatic expression of α-smooth muscle actin and osteopontin. Monocyte therapy is capable of bringing about improvement of liver fibrosis by reducing oxidative stress and inflammation, as well as increasing anti-fibrogenic factors.
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DOI：
10.1016/j.bbrc.2005.08.085
发表时间：
2005-10-14
影响因子：
3.1
作者：
Hung, KS;Lee, TH;Wang, CH
通讯作者：
Wang, CH
影响因子：
4.3
作者：
Elpek, Gulsum Ozlem
通讯作者：
Elpek, Gulsum Ozlem
DOI：
10.1080/00365510701668516
发表时间：
2008-01-01
影响因子：
2.1
作者：
Das, S. K.;Vasudevan, D. M.
通讯作者：
Vasudevan, D. M.
影响因子：
15.9
作者：
Duffield, JS;Forbes, SJ;Iredale, JP
通讯作者：
Iredale, JP
影响因子：
7.4
作者：
BERGMAN, I;LOXLEY, R
通讯作者：
LOXLEY, R