Deciphering the multifactorial nature of Acinetobacter baumannii pathogenicity.
Deciphering the multifactorial nature of Acinetobacter baumannii pathogenicity.
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DOI：
10.1371/journal.pone.0022674
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Visca P
中科院分区：
文献类型：
--
作者：
Antunes LC;Imperi F;Carattoli A;Visca P
鲍曼不动杆菌是一种新兴的细菌病原体，可引起广泛的感染，特别是在住院患者中。近年来，国内外对该菌的流行病学和耐药性研究较多.鲍曼不动杆菌，但目前对其毒力潜力知之甚少。本工作的目的是分析一些与毒力相关的性状。不同来源和临床影响的鲍曼不动杆菌菌株的完整基因组序列，以初步确定新的决定因素。鲍曼不动杆菌致病性临床菌株在大蜡螟感染模型中表现出相当的毒力，无论其状态为暴发或散发菌株，而非人分离株是无毒力的。基因组和表型分析相结合的方法导致了几个毒力因子的鉴定，包括具有溶血、磷脂酶、蛋白酶和铁螯合活性的外产物，以及许多多因子表型，如生物膜形成、表面运动和胁迫抗性，这些因子在A.鲍曼不动杆菌致病性这项工作提供了证据的多因子性质的A。鲍曼不动杆菌毒力而A.鲍曼不动杆菌临床分离株可以代表一个选择的适应于感染人类宿主的菌株群体，高度基因型和表型多样性的A.鲍曼不动杆菌可能存在于医院外环境，其相关性和分布情况值得进一步研究。
Acinetobacter baumannii is an emerging bacterial pathogen that causes a broad array of infections, particularly in hospitalized patients. Many studies have focused on the epidemiology and antibiotic resistance of A. baumannii, but little is currently known with respect to its virulence potential. The aim of this work was to analyze a number of virulence-related traits of four A. baumannii strains of different origin and clinical impact for which complete genome sequences were available, in order to tentatively identify novel determinants of A. baumannii pathogenicity. Clinical strains showed comparable virulence in the Galleria mellonella model of infection, irrespective of their status as outbreak or sporadic strains, whereas a non-human isolate was avirulent. A combined approach of genomic and phenotypic analyses led to the identification of several virulence factors, including exoproducts with hemolytic, phospholipase, protease and iron-chelating activities, as well as a number of multifactorial phenotypes, such as biofilm formation, surface motility and stress resistance, which were differentially expressed and could play a role in A. baumannii pathogenicity. This work provides evidence of the multifactorial nature of A. baumannii virulence. While A. baumannii clinical isolates could represent a selected population of strains adapted to infect the human host, subpopulations of highly genotypically and phenotypically diverse A. baumannii strains may exist outside the hospital environment, whose relevance and distribution deserve further investigation.
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DOI：
10.1083/jcb.147.3.683
发表时间：
1999-11-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Blocker A;Gounon P;Larquet E;Niebuhr K;Cabiaux V;Parsot C;Sansonetti P
通讯作者：
Sansonetti P
影响因子：
3.2
作者：
Loehfelm, Thomas W.;Luke, Nicole R.;Campagnari, Anthony A.
通讯作者：
Campagnari, Anthony A.
影响因子：
3.4
作者：
Choi, CH;Lee, EY;Lee, JC
通讯作者：
Lee, JC
影响因子：
3.7
作者：
de Breij A;Dijkshoorn L;Lagendijk E;van der Meer J;Koster A;Bloemberg G;Wolterbeek R;van den Broek P;Nibbering P
通讯作者：
Nibbering P
影响因子：
--
作者：
Landman, D;Quale, JM;Brooks, S
通讯作者：
Brooks, S