Simultaneous fMRI and local field potential measurements during epileptic seizures in medetomidine-sedated rats using raser pulse sequence.
Simultaneous fMRI and local field potential measurements during epileptic seizures in medetomidine-sedated rats using raser pulse sequence.
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DOI：
10.1002/mrm.22508
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发表时间：
2010-10
影响因子：
3.3
通讯作者：
Grohn, Olli
中科院分区：
文献类型：
--
作者：
Airaksinen, Antti M.;Niskanen, Juha-Pekka;Chamberlain, Ryan;Huttunen, Joanna K.;Nissinen, Jan;Garwood, Michael;Pitkanen, Asia;Grohn, Olli
同时电生理和功能磁共振成像（fMRI）测量癫痫动物模型在方法上具有挑战性，但对于更好地了解癫痫发作期间的异常脑活动和血流动力学至关重要。在本研究中，采用新型快速采集顺序激发和再聚焦（RASER）快速成像脉冲序列和同步局部场电位（LFP）测量，对美托咪定镇静大鼠在kainic酸（KA）诱导的癫痫发作期间进行fMRI。回声平面成像（echo planar imaging， EPI）图像明显可见海马测量电极引起的图像畸变，而激光成像（RASER）图像未见伪影。在KA诱导的癫痫发作期间，海马体观察到强劲的血氧水平依赖性（BOLD）反应。KA注射后LFP信号可检测到复发性癫痫发作。在美托咪定麻醉下，深度电极LFP测量和功能磁共振成像（fMRI）相结合，对KA诱导的癫痫发作没有明显的抑制作用，为研究大鼠的异常脑活动提供了一个独特的工具。
Simultaneous electrophysiological and functional magnetic resonance imaging (fMRI) measurements of animal models of epilepsy are methodologically challenging, but essential to better understand abnormal brain activity and hemodynamics during seizures. In the present study, fMRI of medetomidine sedated rats was performed using novel Rapid Acquisition by Sequential Excitation and Refocusing (RASER) fast imaging pulse sequence and simultaneous local field potential (LFP) measurements during kainic acid (KA) induced seizures. The image distortion caused by the hippocampal measuring electrode was clearly seen in echo planar imaging (EPI) images, whereas no artifact was seen in RASER images. Robust blood oxygenation level dependent (BOLD) responses were observed in the hippocampus during KA induced seizures. The recurrent epileptic seizures were detected in the LFP signal after KA injection. The presented combination of deep electrode LFP measurements and fMRI under medetomidine anesthesia, that does not significantly suppress KA induced seizures, provides a unique tool for studying abnormal brain activity in rats.
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DOI：
10.1111/j.1460-9568.2007.05865.x
发表时间：
2007-11
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Boumans T;Theunissen FE;Poirier C;Van Der Linden A
通讯作者：
Van Der Linden A
DOI：
10.1097/01.wcb.0000131669.02027.3e
发表时间：
2004-09-01
影响因子：
6.3
作者：
Nersesyan, H;Herman, P;Blumenfeld, H
通讯作者：
Blumenfeld, H
影响因子：
2.1
作者：
UEKI, M;MIES, G;HOSSMANN, KA
通讯作者：
HOSSMANN, KA
影响因子：
5.7
作者：
Hara, K;Harris, RA
通讯作者：
Harris, RA
影响因子：
5.6
作者：
Tenney, JR;Duong, TQ;Ferris, CF
通讯作者：
Ferris, CF