A second informational suppressor, SUP-7 X, in Caenorhabditis elegans.
A second informational suppressor, SUP-7 X, in Caenorhabditis elegans.
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秀丽隐杆线虫中的第二个信息抑制因子 SUP-7 X。
DOI：
10.1093/genetics/97.2.307
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发表时间：
1981
期刊：
影响因子：
3.3
通讯作者：
Waterston,RH
中科院分区：
文献类型：
--
作者：
Waterston,RH
在线虫C中已经获得了30多个独立的抑制突变。通过对两个突变体的回复突变分析，许多新的分离株映射到先前鉴定的信息抑制基因sup-5 III的区域（Waterston和Brenner 1978）。其他几个抑制突变定位于X连锁群的左半部分，并定义了第二个抑制基因，sup-7 X。在针对6个基因的40个突变的测试中，发现up-7（st 5）等位基因在更大程度上抑制了up-5（e1464）作用的相同等位基因。Likesup-5（e1464）、sup-7（st 5）作用于肌球蛋白重链基因unc-54 I（MacLeodet al.1977;麦克劳德，Waterston and Brenner 1977）和推定的副肌球蛋白基因unc-15 I（Waterstonet al.1977）的无效等位基因。对func-15（e1214）; sup-7（st 5）动物的化学分析表明，副肌球蛋白恢复到野生型水平的30%以上。- 如对sup-5（e1464）观察到的，sup-7（st 5）的抑制具有剂量依赖性，并且在15°下生长的动物中比在25°下生长的动物中更大。然而，与这种增加的抑制相关的是sup-7（st 5）纯合子的活力降低。致死性的逆转导致了缺陷突变的分离，这些缺陷突变补充了致死性，但缺乏抑制功能。sup-7（st 5）的这些特性表明，它与up-5（e1464）一样，是无效等位基因的信息抑制因子，而其通过缺陷的逆转进一步缩小了对其作用的可能解释。
More than 30 independent suppressor mutations have been obtained in the nematodeC. elegansthrough reversion analysis of twounc-13mutants. Many of the new isolates map to the region of the previously identified informational suppressor,sup-5 III(Waterston and Brenner 1978). Several of the other suppressor mutations map to the left half of theX-linkage group and define a second suppressor gene,sup-7 X. In tests against 40 mutations in six genes, thesup-7(st5)allele was found to suppress to a greater extent the same alleles acted on bysup-5(e1464). Likesup-5(e1464), sup-7(st5)acts on null alleles of the myosin heavy-chain geneunc-54 I(MacLeodet al.1977; MacLeod, Waterston and Brenner 1977) and the putative paramyosin geneunc-15 I(Waterstonet al.1977). Chemical analysis ofunc-15(e1214); sup-7(st5)animals show that paramyosin is restored to more than 30% of the wild-type level. —As was observed forsup-5(e1464), suppression bysup-7(st5)is dose dependent and is greater in animals grown at 15° than at 25°. However, associated with this increased suppression is a decreased viability ofsup-7(st5)homozygotes. Reversion of the lethality has resulted in the isolation of deficiency mutations that complementst5lethality, but lack suppressor function. These properties ofsup-7(st5)suggest that it, likesup-5(e1464), is an informational suppressor of null alleles, and its reversionviadeficiencies further narrows the possible explanations of its action.
影响因子：
5.6
作者：
MACLEOD, AR;WATERSTON, RH;BRENNER, S
通讯作者：
BRENNER, S