Interaction of bile salts with calcium hydroxyapatite: Inhibitors of apatite formation exhibit high–affinity premicellar binding
Interaction of bile salts with calcium hydroxyapatite: Inhibitors of apatite formation exhibit high–affinity premicellar binding
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胆汁盐与羟基磷灰石钙的相互作用：磷灰石形成抑制剂表现出高亲和力的前胶束结合
DOI：
10.1002/hep.1840160526
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发表时间：
1992
期刊：
影响因子：
13.5
通讯作者：
R. Crowther
中科院分区：
文献类型：
--
作者：
Suimmin Qiu;R. Soloway;R. Crowther
在主要的人体胆汁盐中，只有甘氨酸结合的二羟基物种阻止无定形磷酸钙转化为羟基磷灰石钙，羟基磷灰石是胆结石的一种成分;我们提出这种抑制作用是通过胆汁盐和HPO 42 −阴离子之间竞争磷灰石晶体胚胎上的结合位点而发生的。现在，我们表明胆汁盐与完全成熟的羟基磷灰石的结合亲和力具有以下顺序：甘氨酸共轭二羟基盐>牛磺酸共轭二羟基盐>甘氨胆酸盐-牛磺胆酸盐。甘氨酸结合二羟基胆汁盐以高亲和力结合为“预胶束”聚集体，但其余物质似乎以更宽范围的聚集体大小结合。随着pH值从6.6增加到9.8和磷灰石表面电荷从净正向净负反转，Glycochenodeoxycholate结合减少。HPO 42-竞争性抑制结合，但H2 PO 4-不抑制结合。Ca 2+促进了甘氨鹅脱氧胆酸盐，牛磺鹅脱氧胆酸盐和甘氨胆酸盐的结合，后两种胆汁盐的增加与增强的“胶束前”结合。牛磺胆酸盐的结合不受Ca ~（2+）的影响。当将甘氨胆酸盐或牛磺胆酸盐与甘氨鹅脱氧胆酸盐混合时，形成了混合聚集体，其对磷灰石的亲和力低于纯甘氨鹅脱氧胆酸盐聚集体。因为只有甘氨酸结合的二羟基胆汁盐抑制磷灰石的形成，这些结果表明，抑制依赖于高亲和力的“预胶束”胆汁盐-磷灰石结合。（《肝脏学》1992年;16：1280-1289）
Of the major human bile salts, only the glycineconjugated dihydroxy species prevent the transformation of amorphous calcium phosphate to calcium hydroxyapatite, a component of gallstones; we have proposed that this inhibition occurs by competition between the bile salt and HPO42− anions for binding site on the apatite crystal embryo. Now we show that the binding affinity of bile salts to fully mature hydroxyapatite has the following order: glycineconjugated dihydroxy salts > taurine‐conjugated dihydroxy salts > glycocholate ∼ taurocholate. Glycine‐conjugated dihydroxy bile salts bound with high affinity as “premicellar” aggregates, but the remaining species appeared to bind as a wider range of aggregate sizes. Glycochenodeoxycholate binding was decreased as the pH increased from 6.6 to 9.8 and the apatite surface charge reversed from net positive to net negative. Binding was competitively inhibited by HPO42−, but not by H2PO4−. Ca2+ promoted the binding of glycochenodeoxycholate, taurochenodeoxycholate and glycocholate, and for the latter two bile salts the increase was associated with enhanced “premicellar” binding. The binding of taurocholate was not influenced by Ca2+. When either glycocholate or taurocholate was mixed with glycochenodeoxycholate, mixed aggregates were formed that had a lower affinity for apatite than had pure glycochenodeoxycholate aggregates. Because only glycine‐conjugated dihydroxy bile salts inhibit apatite formation, these results suggest that inhibition depends on high‐affinity “premicellar” bile salt–apatite binding. (HEPATOLOGY 1992;16:1280–1289.)
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影响因子：
6.5
作者：
S. Rossi;J. L. Converse;A. Hofmann
通讯作者：
S. Rossi;J. L. Converse;A. Hofmann
DOI：
10.1016/s0021-9258(18)32418-9
发表时间：
1983-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Roda;A. Hofmann;K. J. Mysels
通讯作者：
A. Roda;A. Hofmann;K. J. Mysels
影响因子：
2.9
作者：
CHATTOPADHYAY, A;LONDON, E
通讯作者：
LONDON, E
甘氨鹅去氧胆酸通过阻止无定形磷酸钙转化为羟基磷灰石钙来抑制体外磷酸钙沉淀。
DOI：
10.1172/jci115430
发表时间：
1991
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Qiu,SM;Wen,G;Hirakawa,N;Soloway,RD;Hong,NK;Crowther,RS
通讯作者：
Crowther,RS