Exploring New Methods to Study and Moderate Proton Beam Damage for Multimodal Imaging on a Single Tissue Section.
Exploring New Methods to Study and Moderate Proton Beam Damage for Multimodal Imaging on a Single Tissue Section.
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DOI：
10.1021/jasms.2c00226
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发表时间：
2022-12-07
影响因子：
3.2
通讯作者：
Bailey, Melanie Jane
中科院分区：
文献类型：
--
作者：
Costa, Catia;de Jesus, Janella;Nikula, Chelsea;Murta, Teresa;Grime, Geoffrey W.;Palitsin, Vladimir;Webb, Roger;Goodwin, Richard J. A.;Bunch, Josephine;Bailey, Melanie Jane
表征生物材料中的质子束损伤对于将质子微探针元素测绘技术与其他成像方式相结合是很有意义的。在质子束癌症治疗过程中，对组织中脂质的机械损伤有更深入的了解也是相关的。我们开发了一种新的策略，基于质谱学成像来表征质子束对生物组织中脂质的损伤。这种方法被用来表征体外组织中脂质的变化，这些组织在不同的条件下进行照射，以减轻射线损伤。这项工作表明，在常压下进行质子束照射，以及在照射前应用有机基质，可以减少对组织中脂质的损害。我们还发现，无论质子束辐照，在解吸电喷雾电离成像之前将样品置于真空中可以增强脂质信号，这一结论可能对未来的质谱学成像界有所裨益。
Characterizing proton beam damage in biological materials is of interest to enable the integration of proton microprobe elemental mapping techniques with other imaging modalities. It is also of relevance to obtain a deeper understanding of mechanical damage to lipids in tissues during proton beam cancer therapy. We have developed a novel strategy to characterize proton beam damage to lipids in biological tissues based on mass spectrometry imaging. This methodology is applied to characterize changes to lipids in tissues ex vivo, irradiated under different conditions designed to mitigate beam damage. This work shows that performing proton beam irradiation at ambient pressure, as well as including the application of an organic matrix prior to irradiation, can reduce damage to lipids in tissues. We also discovered that, irrespective of proton beam irradiation, placing a sample in a vacuum prior to desorption electrospray ionization imaging can enhance lipid signals, a conclusion that may be of future benefit to the mass spectrometry imaging community.
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DOI：
10.1021/jasms.0c00232
发表时间：
2020-12-02
影响因子：
3.2
作者：
Neumann, Elizabeth K.;Djambazova, Katerina, V;Caprioli, Richard M.;Spraggins, Jeffrey M.
通讯作者：
Spraggins, Jeffrey M.
影响因子：
3.5
作者：
Canizares, Aurelien;Foucher, Frederic;Westall, Frances
通讯作者：
Westall, Frances
影响因子：
2.9
作者：
Koubenakis, A;Frankevich, V;Zenobi, R
通讯作者：
Zenobi, R
DOI：
10.1016/0168-583x(90)90289-7
发表时间：
1990-04-01
影响因子：
1.3
作者：
LLABADOR, Y;BERTAULT, D;MORETTO, P
通讯作者：
MORETTO, P
影响因子：
15
作者：
Grime, Geoffrey W.;Zeldin, Oliver B.;Garman, Elspeth F.
通讯作者：
Garman, Elspeth F.