UNUSUAL PLASMODIUM MALARIAE-LIKE PARASITES IN SOUTHEAST ASIA
UNUSUAL PLASMODIUM MALARIAE-LIKE PARASITES IN SOUTHEAST ASIA
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东南亚不寻常的类疟原虫寄生虫
DOI：
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发表时间：
2002
影响因子：
1.3
通讯作者：
C. Wongsrichanalai
中科院分区：
文献类型：
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作者：
F. Kawamoto;T. T. Win;S. Mizuno;Khin Lin;O. Kyaw;I. Tantular;D. P. Mason;M. Kimura;C. Wongsrichanalai
在缅甸的疟疾调查期间，发现了两种特殊形式的类似疟疾的疟疾寄生虫。其滋养体早期的形态与典型的间日疟原虫不同，而与间日疟原虫var相似。Emin报道的minuta和Stephens在1914年报道的Tenue疟原虫。两个针对小亚单位核糖体RNA(SSUrRNA)基因相同区域的聚合酶链式反应(PCR)诊断表明，这些寄生虫是疟疾原虫的新变种，可分为两个与这两个形态类型相关的遗传型。对SSUrRNA和环子孢子蛋白基因的序列分析表明，它们既不同于彼此，也不同于其他已知的疟疾分离株，而且类似P.tenue的类型更接近于猴四联疟原虫巴西疟原虫。这些结果表明，人类疟原虫的显微外观可能比先前假设的更不同，并表明分子工具在评估疟疾形态变异中的价值。
During malaria surveys in Myanmar, 2 peculiar forms of Plasmodium malariae-like parasites were found. The morphologies of their early trophozoite stages were distinct from that of the typical P. malariae, resembling instead that of Plasmodium vivax, var. minuta, reported by Emin, and Plasmodium tenue, reported by Stephens, both in 1914. Two polymerase chain reaction (PCR)-based diagnoses, which target the same regions in the small subunit ribosomal RNA (SSUrRNA) genes, indicated that these parasites were new variant forms of P. malariae and that they could be separated into 2 genetic types that correlated with the 2 morphological types. Sequence analysis of the SSUrRNA and the circumsporozoite protein genes revealed that they were distinct both from each other and from other known P. malariae isolates and that the P. tenue-like type was closer to a monkey quartan malaria parasite, Plasmodium brasilianum. These results illustrate that the microscopic appearance of human P. malariae parasites may be more varied than previously assumed and suggest the value of molecular tools in the evaluation of malaria morphological variants.
人类和灵长类疟疾的进化起源：来自环子孢子蛋白基因的证据。
影响因子：
10.7
作者：
Escalante,AA;Barrio,E;Ayala,FJ
通讯作者：
Ayala,FJ