CRISPR-induced miRNA156-recognition element mutations in TaSPL13 improve multiple agronomic traits in wheat.
CRISPR-induced miRNA156-recognition element mutations in TaSPL13 improve multiple agronomic traits in wheat.
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DOI：
10.1111/pbi.13969
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发表时间：
2023-03
影响因子：
13.8
通讯作者：
Li, Wanlong
中科院分区：
文献类型：
--
作者：
Gupta, Ajay;Hua, Lei;Zhang, Zhengzhi;Yang, Bing;Li, Wanlong
提高粮食产量一直是小麦遗传改良的主要目标。SQUAMOSA启动子结合蛋白样（SPL）基因编码了一个小家族的多种植物特异性转录因子，是提高水稻产量和其他主要农艺性状的重要靶点。小麦SPL基因在这方面的功能还有待进一步研究。在这项研究中，我们鉴定了56个小麦同源水稻SPL基因，属于19个同源群。与水稻一样，9个同源TaSPL基因在其最后外显子中含有microRNA156识别元件（MRE），除了TaSPL13，其在其3 ‘‐非翻译区（3 ’ utr）中含有MRE。我们使用CRISPR‐Cas9修饰了TaSPL13的MRE，并在三个同源基因中产生了12个突变。正如预期的那样，MRE突变导致TaSPL13突变体转录物增加了大约两倍。表型评价结果表明，MRE突变导致TaSPL13的开花时间、分蘖数和株高减少，籽粒大小和数量增加。结果表明，TaSPL13突变体具有拟南芥AtSPL3/4/5突变体和水稻OsSPL13/14/16突变体中不同表型的组合，具有通过同时增加籽粒大小和数量以及改善植株结构来提高小麦产量的巨大潜力。所产生的新型TaSPL13突变可用于小麦育种计划，以改善这些农艺性状。
Increase in grain yield is always a major objective of wheat genetic improvement. The SQUAMOSA promoter‐binding protein‐like (SPL) genes, coding for a small family of diverse plant‐specific transcription factors, represent important targets for improving grain yield and other major agronomic traits in rice. The function of the SPL genes in wheat remains to be investigated in this respect. In this study, we identified 56 wheat orthologues of rice SPL genes belonging to 19 homoeologous groups. Like in rice, nine orthologous TaSPL genes harbour the microRNA156 recognition elements (MRE) in their last exons except for TaSPL13, which harbour the MRE in its 3′‐untranslated region (3′UTR). We modified the MRE of TaSPL13 using CRISPR‐Cas9 and generated 12 mutations in the three homoeologous genes. As expected, the MRE mutations led to an approximately two‐fold increase in the TaSPL13 mutant transcripts. The phenotypic evaluation showed that the MRE mutations in TaSPL13 resulted in a decrease in flowering time, tiller number, and plant height, and a concomitantly increase in grain size and number. The results show that the TaSPL13 mutants exhibit a combination of different phenotypes observed in Arabidopsis AtSPL3/4/5 mutants and rice OsSPL13/14/16 mutants and hold great potential in improving wheat yield by simultaneously increasing grain size and number and by refining plant architecture. The novel TaSPL13 mutations generated can be utilized in wheat breeding programmes to improve these agronomic traits.
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影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
通过反式编码的小 RNA 和宿主因子 RNase III 进行 CRISPR RNA 成熟。
影响因子：
64.8
作者：
Deltcheva, Elitza;Chylinski, Krzysztof;Sharma, Cynthia M.;Gonzales, Karine;Chao, Yanjie;Pirzada, Zaid A.;Eckert, Maria R.;Vogel, Joerg;Charpentier, Emmanuelle
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Charpentier, Emmanuelle
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4.3
作者：
Cao J;Liu K;Song W;Zhang J;Yao Y;Xin M;Hu Z;Peng H;Ni Z;Sun Q;Du J
通讯作者：
Du J
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
64.8
作者：
Anzalone, Andrew V.;Randolph, Peyton B.;Liu, David R.
通讯作者：
Liu, David R.