Pleiotropic function of the SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE gene TaSPL14 in wheat plant architecture.
Pleiotropic function of the SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE gene TaSPL14 in wheat plant architecture.
复制标题
DOI：
10.1007/s00425-020-03531-x
复制
发表时间：
2021-01-22
期刊：
影响因子：
4.3
通讯作者：
Du J
中科院分区：
文献类型：
--
作者：
Cao J;Liu K;Song W;Zhang J;Yao Y;Xin M;Hu Z;Peng H;Ni Z;Sun Q;Du J
SQUAMOSA PROMOTER-BINDING PROTEIN-BOX基因TaSPL 14在小麦中的功能与水稻中的OsSPL 14在调节小麦株高、穗长、小穗数和千粒重方面的功能相似，但在分蘖发育中的功能不同。TaSPL 14可能通过乙烯反应基因EIN 3-LIKE 1（TaEIL 1）、乙烯反应转录因子2.11（TaRAP 2.11）和乙烯反应转录因子1（TaERF 1）调控小麦穗的发育，但不通过TaDEP 1调控小麦穗的发育。水稻的SQUAMOSA启动子结合蛋白样基因OsSPL 14被认为是理想植株结构的主要决定因素，该理想植株结构由很少的无效分蘖、每穗更多的粒数和茎对倒伏的高抗性组成。然而，其直向同源基因TaSPL 14在小麦中的功能尚不清楚。在这里，我们报道了TaSPL 14和OsSPL 14之间的功能相似性和差异。与水稻中OsSPL 14基因敲除相似，小麦TaSPL 14基因敲除植物表现出降低的株高、穗长、小穗数和千粒重。然而，与OsSPL 14相反，TaSPL 14不影响分蘖数。转录组分析表明，与野生型相比，TaSPL 14基因敲除株系幼穗中乙烯反应相关基因的表达量显著降低。TaSPL 14与乙烯反应基因TaEIL 1、TaRAP 2.11和TaERF 1的启动子直接结合，促进它们的表达，表明TaSPL 14可能通过乙烯反应途径调控小麦穗的发育。TaSPL 14的阐明将有助于理解小麦植株结构的分子机制。在线版本包含补充材料，可通过10.1007/s 00425 -020-03531-x获得。
The function of SQUAMOSA PROMOTER-BINDING PROTEIN-BOX gene TaSPL14 in wheat is similar to that of OsSPL14 in rice in regulating plant height, panicle length, spikelet number, and thousand-grain weight of wheat, but differs during tiller development. TaSPL14 may regulate spike development via ethylene-response gene EIN3-LIKE 1 (TaEIL1), ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 2.11 (TaRAP2.11), and ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 1 (TaERF1), but not DENSE AND ERECT PANICLE 1 (TaDEP1) in wheat. The SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE gene OsSPL14 from rice is considered to be a major determinant of ideal plant architecture consisting of few unproductive tillers, more grains per spike, and high resistance of stems to lodging. However, the function of its orthologous gene, TaSPL14, in wheat is unknown. Here, we reported the functional similarities and differences between TaSPL14 and OsSPL14. Similar to OsSPL14 knock-outs in rice, wheat TaSPL14 knock-out plants exhibited decreased plant height, panicle length, spikelet number, and thousand-grain weight. In contrast to OsSPL14, however, TaSPL14 did not affect tiller number. Transcriptome analysis revealed that the expression of genes related to ethylene response was significantly decreased in young spikes of TaSPL14 knock-out lines as compared with wild type. TaSPL14 directly binds to the promoters of the ethylene-response genes TaEIL1, TaRAP2.11, and TaERF1, and promotes their expression, suggesting that TaSPL14 might regulate wheat spike development via the ethylene-response pathway. The elucidation of TaSPL14 will contribute to understanding of the molecular mechanisms that underlie wheat plant architecture. The online version contains supplementary material available at 10.1007/s00425-020-03531-x.
登录
查看更多内容
影响因子：
4.9
作者：
Minakuchi K;Kameoka H;Yasuno N;Umehara M;Luo L;Kobayashi K;Hanada A;Ueno K;Asami T;Yamaguchi S;Kyozuka J
通讯作者：
Kyozuka J
影响因子：
9.4
作者：
Li, Lin-Feng;Liu, Bao;Wendel, Jonathan F.
通讯作者：
Wendel, Jonathan F.
影响因子：
7.4
作者：
Feng, Nan;Song, Gaoyuan;Mao, Long
通讯作者：
Mao, Long
影响因子：
64.8
作者：
Peng, JR;Richards, DE;Harberd, NP
通讯作者：
Harberd, NP
影响因子：
5.4
作者：
Kuraparthy, Vasu;Sood, Shilpa;Gill, Bikram S.
通讯作者：
Gill, Bikram S.