MAP1B is required for axon guidance and Is involved in the development of the central and peripheral nervous system.
MAP1B is required for axon guidance and Is involved in the development of the central and peripheral nervous system.
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MAP1B 是轴突引导所必需的，并且参与中枢和周围神经系统的发育。
DOI：
10.1083/jcb.151.6.1169
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发表时间：
2000-12-11
影响因子：
7.8
通讯作者：
Propst, F
中科院分区：
文献类型：
--
作者：
Meixner, A;Haverkamp, S;Wässle, H;Führer, S;Thalhammer, J;Kropf, N;Bittner, RE;Lassmann, H;Wiche, G;Propst, F
长期以来，人们一直怀疑 MAP1B 等微管相关蛋白在神经元分化中发挥重要作用，但一直缺乏证据。之前的 MAP1B 基因靶向研究产生了矛盾且不确定的结果，并且没有揭示 MAP1B 的功能。与之前的两项工作相比，我们现在描述了完整 MAP1B 无效等位基因的生成。 MAP1B 缺失的杂合小鼠不受影响。纯合突变体是可行的，但在大脑中表现出惊人的发育缺陷，胼胝体选择性缺失，并伴随形成由误导的皮质轴突组成的有髓鞘纤维束。此外，MAP1B 缺陷小鼠的周围神经中大有髓轴突的数量减少。其余轴突的髓鞘厚度减少，导致成年坐骨神经的神经传导速度降低。另一方面，MAP1B 参与视网膜发育和 γ-氨基丁酸 C 受体聚集的预期并未得到证实。我们的结果证明了 MAP1B 在神经系统发育和功能中的重要作用，并解决了之前关于其重要性的争议。
Microtubule-associated proteins such as MAP1B have long been suspected to play an important role in neuronal differentiation, but proof has been lacking. Previous MAP1B gene targeting studies yielded contradictory and inconclusive results and did not reveal MAP1B function. In contrast to two earlier efforts, we now describe generation of a complete MAP1B null allele. Mice heterozygous for this MAP1B deletion were not affected. Homozygous mutants were viable but displayed a striking developmental defect in the brain, the selective absence of the corpus callosum, and the concomitant formation of myelinated fiber bundles consisting of misguided cortical axons. In addition, peripheral nerves of MAP1B-deficient mice had a reduced number of large myelinated axons. The myelin sheaths of the remaining axons were of reduced thickness, resulting in a decrease of nerve conduction velocity in the adult sciatic nerve. On the other hand, the anticipated involvement of MAP1B in retinal development and γ-aminobutyric acid C receptor clustering was not substantiated. Our results demonstrate an essential role of MAP1B in development and function of the nervous system and resolve a previous controversy over its importance.
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影响因子：
3.4
作者：
Koulen, P;Brandstätter, JH;Wässle, H
通讯作者：
Wässle, H
影响因子：
16.2
作者：
Lanier, LM;Gates, MA;Gertler, FB
通讯作者：
Gertler, FB
影响因子：
4.4
作者：
LIEN, LL;FEENER, CA;KUNKEL, LM
通讯作者：
KUNKEL, LM
DOI：
10.1073/pnas.93.3.1270
发表时间：
1996-02-06
影响因子：
11.1
作者：
Edelmann, W;Zervas, M;Kucherlapati, R
通讯作者：
Kucherlapati, R
影响因子：
64.8
作者：
Hanley, JG;Koulen, P;Moss, SJ
通讯作者：
Moss, SJ