Afadin: A key molecule essential for structural organization of cell-cell junctions of polarized epithelia during embryogenesis.
Afadin: A key molecule essential for structural organization of cell-cell junctions of polarized epithelia during embryogenesis.
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Afadin：胚胎发生过程中极化上皮细胞-细胞连接结构组织所必需的关键分子。
DOI：
10.1083/jcb.146.5.1117
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发表时间：
1999-09-06
期刊：
影响因子：
--
通讯作者：
Takai Y
中科院分区：
文献类型：
--
作者：
Ikeda W;Nakanishi H;Miyoshi J;Mandai K;Ishizaki H;Tanaka M;Togawa A;Takahashi K;Nishioka H;Yoshida H;Mizoguchi A;Nishikawa S;Takai Y
Afadin是一种肌动蛋白亲和结合蛋白，其与免疫球蛋白样细胞粘附分子nectin结合，并与nectin共定位于基于钙粘蛋白的细胞-细胞粘附连接（AJs）。为了探索afadin在胚胎发生过程中细胞间粘附的功能，我们产生了afadin−/−小鼠和胚胎干细胞。在胚胎6.5-8.5天的野生型小鼠中，afadin在胚胎外胚层和中胚层中高度表达，但在胚外区域如内脏内胚层中几乎检测不到。Afadin−/−小鼠在原肠胚形成期间和之后的阶段表现出发育缺陷，包括外胚层的解体，中胚层的迁移受损，以及体节和来自外胚层和中胚层的其他结构的丢失。来自afadin−/−胚胎干细胞的囊状胚状体显示出内胚层的正常组织，但外胚层的紊乱。在afadin−/−小鼠和胚状体的外胚层中，细胞间AJs和紧密连接组织不当。这些结果表明，afadin在胚胎发生期间在外胚层衍生的细胞中高度表达，并且在AJs的适当组织和高表达细胞的紧密连接中起关键作用，这对于适当的组织形态发生是必需的。
Afadin is an actin filament–binding protein that binds to nectin, an immunoglobulin-like cell adhesion molecule, and is colocalized with nectin at cadherin-based cell–cell adherens junctions (AJs). To explore the function of afadin in cell–cell adhesion during embryogenesis, we generated afadin−/− mice and embryonic stem cells. In wild-type mice at embryonic days 6.5–8.5, afadin was highly expressed in the embryonic ectoderm and the mesoderm, but hardly detected in the extraembryonic regions such as the visceral endoderm. Afadin−/− mice showed developmental defects at stages during and after gastrulation, including disorganization of the ectoderm, impaired migration of the mesoderm, and loss of somites and other structures derived from both the ectoderm and the mesoderm. Cystic embryoid bodies derived from afadin−/− embryonic stem cells showed normal organization of the endoderm but disorganization of the ectoderm. Cell–cell AJs and tight junctions were improperly organized in the ectoderm of afadin−/− mice and embryoid bodies. These results indicate that afadin is highly expressed in the ectoderm- derived cells during embryogenesis and plays a key role in proper organization of AJs and tight junctions of the highly expressing cells, which is essential for proper tissue morphogenesis.
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影响因子：
7.8
作者：
Buchert, M;Schneider, S;Meskenaite, V;Adams, M T;Canaani, E;Baechi, T;Moelling, K;Hovens, C M
通讯作者：
Hovens, C M
DOI：
10.1083/jcb.121.3.491
发表时间：
1993-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Itoh M;Nagafuchi A;Yonemura S;Kitani-Yasuda T;Tsukita S;Tsukita S
通讯作者：
Tsukita S
Afadin：一种新型的肌动蛋白结合蛋白，其中一个PDZ结构域位于基于钙粘蛋白的细胞到细胞粘附连接处。
影响因子：
7.8
作者：
Mandai, K;Nakanishi, H;Satoh, A;Obaishi, H;Wada, M;Nishioka, H;Itoh, M;Mizoguchi, A;Aoki, T;Fujimoto, T;Matsuda, Y;Tsukita, S;Takai, Y
通讯作者：
Takai, Y
DOI：
10.1083/jcb.141.7.1539
发表时间：
1998-06-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Furuse M;Fujita K;Hiiragi T;Fujimoto K;Tsukita S
通讯作者：
Tsukita S
影响因子：
64.5
作者：
NAGAFUCHI, A;TAKEICHI, M;TSUKITA, S
通讯作者：
TSUKITA, S