Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction.
Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction.
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Afadin：一种新型的肌动蛋白结合蛋白，其中一个PDZ结构域位于基于钙粘蛋白的细胞到细胞粘附连接处。
DOI：
10.1083/jcb.139.2.517
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发表时间：
1997-10-20
影响因子：
7.8
通讯作者：
Takai, Y
中科院分区：
文献类型：
--
作者：
Mandai, K;Nakanishi, H;Satoh, A;Obaishi, H;Wada, M;Nishioka, H;Itoh, M;Mizoguchi, A;Aoki, T;Fujimoto, T;Matsuda, Y;Tsukita, S;Takai, Y
分离并鉴定了一种新的肌动蛋白结合蛋白(F-肌动蛋白)，其分子质量为∼205kD(P205)，主要集中在钙粘附素的细胞-细胞黏附连接(AJ)处。从大鼠脑中分离纯化P205，并从大鼠脑cDNA文库中克隆了P205的全长基因。P205蛋白由1,829个氨基酸组成，分子量为207,667 kD。P205在1,631-1,829个氨基酸残基上有一个F-肌动蛋白结合域，在1,016-1,100个氨基酸残基上有一个PDZ结构域，该结构域与跨膜蛋白相互作用。P205与另一种相对分子质量为190kD(P190)的蛋白从大鼠脑组织中融合。P190是一个由1,663个氨基酸组成的蛋白质，计算的相对分子质量为188,971 kD。P190是p205的一个剪接变异体，在1,009-1,093个氨基酸残基上有一个PDZ结构域，但缺少F-肌动蛋白结合域。同源性搜索分析表明，p190的AA序列与AF-6基因的产物在整个序列上有90%的同源性，该基因被发现与与急性白血病有关的ALL-1基因融合。P190很可能是人类AF-6蛋白的大鼠对应体。P205沿F-肌动蛋白的两侧结合，但几乎不显示F-肌动蛋白的交联活性。Northern和Western印迹分析表明，p205在所有被检测的大鼠组织中普遍表达，而p190在脑中特异表达。免疫荧光和免疫电子显微镜研究表明，p205集中在不同组织中以钙粘附素为基础的细胞间结合。我们命名为p205 L-afadin(定位于粘连连接的AF-6蛋白的大剪接变异体)和p190 S-afadin(L-afadin的小剪接变异体)。这些结果表明，L-阿法丁在细胞与细胞间的结合中是肌动蛋白细胞骨架与质膜的连接物。
A novel actin filament (F-actin)–binding protein with a molecular mass of ∼205 kD (p205), which was concentrated at cadherin-based cell-to-cell adherens junction (AJ), was isolated and characterized. p205 was purified from rat brain and its cDNA was cloned from a rat brain cDNA library. p205 was a protein of 1,829 amino acids (aa) with a calculated molecular mass of 207,667 kD. p205 had one F-actin–binding domain at 1,631–1,829 aa residues and one PDZ domain at 1,016– 1,100 aa residues, a domain known to interact with transmembrane proteins. p205 was copurified from rat brain with another protein with a molecular mass of 190 kD (p190). p190 was a protein of 1,663 aa with a calculated molecular mass of 188,971 kD. p190 was a splicing variant of p205 having one PDZ domain at 1,009–1,093 aa residues but lacking the F-actin–binding domain. Homology search analysis revealed that the aa sequence of p190 showed 90% identity over the entire sequence with the product of the AF-6 gene, which was found to be fused to the ALL-1 gene, known to be involved in acute leukemia. p190 is likely to be a rat counterpart of human AF-6 protein. p205 bound along the sides of F-actin but hardly showed the F-actin–cross-linking activity. Northern and Western blot analyses showed that p205 was ubiquitously expressed in all the rat tissues examined, whereas p190 was specifically expressed in brain. Immunofluorescence and immunoelectron microscopic studies revealed that p205 was concentrated at cadherin-based cell-to-cell AJ of various tissues. We named p205 l-afadin (a large splicing variant of AF-6 protein localized at adherens junction) and p190 s-afadin (a small splicing variant of l-afadin). These results suggest that l-afadin serves as a linker of the actin cytoskeleton to the plasma membrane at cell-to-cell AJ.
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影响因子：
5.7
作者：
Bentley, David;O'Connor, Timothy P.
通讯作者：
O'Connor, Timothy P.
DOI：
10.1083/jcb.121.3.491
发表时间：
1993-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Itoh M;Nagafuchi A;Yonemura S;Kitani-Yasuda T;Tsukita S;Tsukita S
通讯作者：
Tsukita S
影响因子：
64.8
作者：
KIM, E;NIETHAMMER, M;SHENG, M
通讯作者：
SHENG, M
DOI：
10.1083/jcb.115.5.1449
发表时间：
1991-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Itoh M;Yonemura S;Nagafuchi A;Tsukita S;Tsukita S
通讯作者：
Tsukita S
影响因子：
7.5
作者：
Geiger, B.
通讯作者：
Geiger, B.