Establishment of six human pancreatic cancer cell lines and their sensitivities to anti-tumor drugs.
Establishment of six human pancreatic cancer cell lines and their sensitivities to anti-tumor drugs.
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六种人胰腺癌细胞系的建立及其对抗肿瘤药物的敏感性。
DOI：
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发表时间：
1986
影响因子：
2.2
通讯作者：
T. Tachibana
中科院分区：
文献类型：
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作者：
M. Kobari;H. Hisano;Seiki Matsuno;Toshio Sato;M. Kan;T. Tachibana
建立了6株人胰腺癌细胞系PK-1、-8、-9、-12、-14和-16。它们来自原发胰腺癌活检或肝转移活检，或这些活检标本在裸鼠体内的异种移植。原发肿瘤均为胰管来源的高分化腺癌。所建立的6株PK细胞系均为CEA阳性，在裸鼠体内具有致瘤性。移植瘤的形态与原始肿瘤非常相似。PK细胞生长缓慢，倍增时间为41.3~82小时，染色体呈非整倍体。细胞提取液中葡萄糖-6-磷酸脱氢酶(G6PDH)和乳酸脱氢酶(LDH)含量较高。除PK-8和PK-9外，细胞提取液中未检测到胰蛋白酶。在药敏试验中，所有PK细胞株对阿克拉霉素A(ACM)均敏感，PK-1和PK-8对浓度为0.02微克/毫升的5-氟尿嘧啶(5-FU)和1微克/毫升的5-氟尿嘧啶(5-FU)敏感。在较高浓度下，它们对丝裂霉素C(MMC)表现出时间依赖性的敏感性。PK-9对5-FU和MMC耐药。
Six human pancreatic cancer cell lines PK-1, -8, -9, -12, -14 and -16, were established. They originated from either primary pancreatic cancer biopsy or liver metastasis biopsy, or xenografts of these biopsy specimens in athymic nude mice. The primary tumors were all well differentiated adenocarcinomas of pancreatic duct origin. The six established PK cell lines were all CEA positive and had tumorigenicity in athymic nude mice. Morphology of the xenografted tumors was closely similar to that of the original tumor. PK cells grew slowly with the doubling time of 41.3 to 82 hr and showed aneuploid chromosome pattern. High levels of glucose-6-phosphate dehydrogenase (G6PDH) and lactic dehydrogenase (LDH) were found in each cell extract. Trypsin was not detected in cell extracts except PK-8 and PK-9. In chemosensitivity test, all of PK cell lines were sensitive to aclacinomycin A (ACM), and PK-1 and PK-8 were sensitive to 5-Fluorouracil (5-Fu) at concentrations of 0.02 microgram/ml, ACM and 1 microgram/ml, 5-Fu, when the drugs were used for over 48 hr. At higher concentrations, they showed time independent sensitivity to mitomycin C (MMC). PK-9 was resistant to 5-Fu and MMC.
评估两种胰腺腺癌细胞系细胞毒活性的体外方法的比较。
影响因子：
11.2
作者：
Chang,BK
通讯作者：
Chang,BK
无胸腺小鼠中两种致瘤性人胰腺癌细胞系的建立和表征。
影响因子：
11.2
作者：
Dexter,DL;Matook,GM;Meitner,PA;Bogaars,HA;Jolly,GA;Turner,MD;Calabresi,P
通讯作者：
Calabresi,P
影响因子：
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作者：
Konno, Hayato;Minamiya, Yoshihiro;Ogawa, Jun-ichi
通讯作者：
Ogawa, Jun-ichi