A novel onsite and visual molecular technique to authenticate saffron (Crocus sativus) and its adulterants based on recombinase polymerase amplification
A novel onsite and visual molecular technique to authenticate saffron (Crocus sativus) and its adulterants based on recombinase polymerase amplification
复制标题
基于重组酶聚合酶扩增的新型现场视觉分子技术鉴定藏红花（Crocus sativus）及其掺假品
DOI：
10.1016/j.foodcont.2019.01.011
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发表时间：
2019-06
期刊：
影响因子：
6
通讯作者：
Sun Wei
中科院分区：
文献类型：
--
作者：
Zhao Mingming;Wang Bo;Xiang Li;Xiong Chao;Shi Yuhua;Wu Lan;Meng Xiangxiao;Dong Gangqiang;Xie Yiqiang;Sun Wei
藏红花(西红花)是中国笔下最贵的药材之一，由于价格高，产量有限，一直受到各种类型的掺假。本工作描述了一种灵敏、高效和简单的现场鉴定藏红花的技术。采用重组酶聚合酶扩增(RPA)结合通用侧向流动免疫分析纸条(RPA-LFD)对藏红花进行快速目视检测。该方法能在37 °C下，在15 分钟内检测到藏红花，灵敏度高，检出限为1 PG基因组DNA，具有较高的特异性，与常见的藏红花混伪品无交叉反应。这一简单而灵敏的方法非常适合于藏红花的快速分子检测，结果是条带的存在(阳性条带)或不存在(无阳性条带)。
Saffron (Crocus sativus), one of the most expensive herbs in China, has been subject to various types of adulteration as a consequence of its high price and limited production. The present work describes a sensitive, efficient and simple technique for on-site saffron identification. Recombinase polymerase amplification (RPA) in combination with a universal lateral flow immunoassay strip (saffron RPA-LFD) was employed for rapid visual detection of saffron. This assay was able to detect saffron in less than 15 min at 37 °C. This assay was highly sensitive with detection limits of 1 pg genomic DNA, and highly specific, with no cross-reaction with common saffron adulterants. This proposed simple and sensitive assay is well-suited for rapid molecular detection of saffron, with results in the form of the presence (positive bands) or absence (no positive bands) of bands.
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影响因子：
--
作者：
Jiang C;Cao L;Yuan Y;Chen M;Jin Y;Huang L
通讯作者：
Huang L
影响因子：
5.6
作者：
Liu W;Liu HX;Zhang L;Hou XX;Wan KL;Hao Q
通讯作者：
Hao Q
影响因子：
2.8
作者：
Askary Vahid Reza;Dourandish Nazanin;S. Athena;Jahan Nia Arman;J. Saeed;Khosand Kamachali Ali Reza
通讯作者：
Askary Vahid Reza;Dourandish Nazanin;S. Athena;Jahan Nia Arman;J. Saeed;Khosand Kamachali Ali Reza
影响因子：
4.6
作者：
Zhao M;Shi Y;Wu L;Guo L;Liu W;Xiong C;Yan S;Sun W;Chen S
通讯作者：
Chen S
使用重组酶聚合酶扩增测定快速鉴定银杏草药产品
影响因子：
4.6
作者：
Liu Y;Wang XY;Wei XM;Gao ZT;Han JP
通讯作者：
Han JP