Barcoding melting curve analysis for rapid, sensitive, and discriminating authentication of saffron (Crocus sativus L.) from its adulterants.
Barcoding melting curve analysis for rapid, sensitive, and discriminating authentication of saffron (Crocus sativus L.) from its adulterants.
复制标题
DOI：
10.1155/2014/809037
复制
发表时间：
2014
影响因子：
--
通讯作者：
Huang L
中科院分区：
文献类型：
--
作者：
Jiang C;Cao L;Yuan Y;Chen M;Jin Y;Huang L
藏红花（Crocus sativus L.）是世界上最重要和最昂贵的药用香料产品之一。由于其高市场价值和高价，藏红花经常通过掺入其他材料来掺假，例如红花和金盏花、萱草花瓣、胡萝卜肉质根、姜黄根茎、玉米和荷花。耻辱。为了开发一种简单、非测序的方法来快速、灵敏和有区别地检测贸易藏红花中的这些掺假物，我们在此报告了条形码熔解曲线分析方法 (Bar-MCA) 的应用，该方法使用通用叶绿体植物 DNA 条形码区域 trnH-psbA 来识别掺假物。当在针对曲线分析优化的 DNA 浓度和退火温度下进行扩增时，藏红花 (81.92°C) 和掺假品：D. carota (81.60°C)、C.tinctorius (80.10°C)、C. officinalis (79.92°C)、Dendranthema morifolium (Ramat.) Tzvel 在特定位置形成峰。 (79.62°C)、N. nucifera (80.58°C)、Hemerocallis fulva (L.) L. (84.78°C) 和 Z. mays (84.33°C)。所构建的藏红花及其掺杂物的熔解曲线具有显着不同的峰位置或形状。总之，Bar-MCA 可能是一种更快、更具成本效益的藏红花鉴定和检测掺假品的方法。
Saffron (Crocus sativus L.) is one of the most important and expensive medicinal spice products in the world. Because of its high market value and premium price, saffron is often adulterated through the incorporation of other materials, such as Carthamus tinctorius L. and Calendula officinalis L. flowers, Hemerocallis L. petals, Daucus carota L. fleshy root, Curcuma longa L. rhizomes, Zea may L., and Nelumbo nucifera Gaertn. stigmas. To develop a straightforward, nonsequencing method for rapid, sensitive, and discriminating detection of these adulterants in traded saffron, we report here the application of a barcoding melting curve analysis method (Bar-MCA) that uses the universal chloroplast plant DNA barcoding region trnH-psbA to identify adulterants. When amplified at DNA concentrations and annealing temperatures optimized for the curve analysis, peaks were formed at specific locations for saffron (81.92°C) and the adulterants: D. carota (81.60°C), C. tinctorius (80.10°C), C. officinalis (79.92°C), Dendranthema morifolium (Ramat.) Tzvel. (79.62°C), N. nucifera (80.58°C), Hemerocallis fulva (L.) L. (84.78°C), and Z. mays (84.33°C). The constructed melting curves for saffron and its adulterants have significantly different peak locations or shapes. In conclusion, Bar-MCA could be a faster and more cost-effective method to authenticate saffron and detect its adulterants.
登录
查看更多内容
影响因子：
8.1
作者：
Maggi, Luana;Carmona, Manuel;Alonso, Gonzalo L.
通讯作者：
Alonso, Gonzalo L.
影响因子：
4.3
作者：
Lage, Mounira;Cantrell, Charles L.
通讯作者：
Cantrell, Charles L.
影响因子：
6
作者：
Marieschi, Matteo;Torelli, Anna;Bruni, Renato
通讯作者：
Bruni, Renato
影响因子：
6
作者：
Babaei, Saeid;Talebi, Majid;Bahar, Masoud
通讯作者：
Bahar, Masoud
DOI：
10.1073/pnas.0812297106
发表时间：
2009-02-24
影响因子：
11.1
作者：
Awano, Tomoyuki;Johnson, Gary S.;Coates, Joan R.
通讯作者：
Coates, Joan R.