Novel Functions of the Fatty Acid and Retinol Binding Protein (FAR) Gene Family Revealed by Fungus-Mediated RNAi in the Parasitic Nematode, Aphelenchoides besseyi.
Novel Functions of the Fatty Acid and Retinol Binding Protein (FAR) Gene Family Revealed by Fungus-Mediated RNAi in the Parasitic Nematode, Aphelenchoides besseyi.
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真菌介导的 RNAi 介导的寄生线虫中脂肪酸和视黄醇结合蛋白 (FAR) 基因家族的新功能
DOI：
10.3390/ijms221810057
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发表时间：
2021-09-17
影响因子：
5.6
通讯作者：
Xie H
中科院分区：
文献类型：
--
作者：
Ding S;Xu C;Chen C;Li J;Wang J;Xie H
RNA干扰（RNAi）是分析线虫基因功能的有力工具。脂肪酸与视黄醇结合蛋白（Fatty acid and retinol binding protein， FAR）是一种仅存在于线虫体内的蛋白，在线虫的生命活动中起着重要作用。水稻白尖线虫（Aphelenchoides besseyi）是一种迁移性植物内寄生线虫，对农业生产造成严重危害。在本研究中，RWTN通过饲喂Ab-far-n (n: 1-8) hairpin RNA转基因Botrytis cinerea （ARTBn）有效降低了8个RWTN基因的表达水平。通过任何基因沉默后的表型变化，发现远基因家族的这些功能是一致和多样的。这种一致性表明，在沉默8个Ab-far基因中的任何一个后，雌性的体长都显着缩短。其多样性主要表现在：(1)对Ab-far-1 ~ Ab-far-4进行沉默处理后，线虫的繁殖受到明显抑制。此外，沉默Ab-far-2可以抑制线虫对拟南芥的致病性；(2)沉默Ab-far-2和Ab-far-4后，雌线虫性腺长度明显缩短；(3)沉默Ab-far-1、Ab-far-3和Ab-far-5后，成虫种群中雄虫比例显著增加，而沉默Ab-far-4后，成虫比例显著降低。(4)沉默Ab-far-3、Ab-far-4和Ab-far-7后，线虫的脂肪储存显著降低。据我们所知，这是首次证实Ab-far基因影响线虫性别形成和脂质代谢的研究，为进一步研究和控制RWTNs提供了有价值的数据。
RNA interference (RNAi) is a powerful tool for the analysis of gene function in nematodes. Fatty acid and retinol binding protein (FAR) is a protein that only exists in nematodes and plays an important role in their life activities. The rice white-tip nematode (RWTN), Aphelenchoides besseyi, is a migratory endoparasitic plant nematode that causes serious damage in agricultural production. In this study, the expression levels of eight RWTN genes were effectively decreased when RWTN was fed Ab-far-n (n: 1–8) hairpin RNA transgenic Botrytis cinerea (ARTBn). These functions of the far gene family were identified to be consistent and diverse through phenotypic changes after any gene was silenced. Such consistency indicates that the body lengths of the females were significantly shortened after silencing any of the eight Ab-far genes. The diversities were mainly manifested as follows: (1) Reproduction of nematodes was clearly inhibited after Ab-far-1 to Ab-far-4 were silenced. In addition, silencing Ab-far-2 could inhibit the pathogenicity of nematodes to Arabidopsis; (2) gonad length of female nematodes was significantly shortened after Ab-far-2 and Ab-far-4 were silenced; (3) proportion of male nematodes significantly increased in the adult population after Ab-far-1, Ab-far-3, and Ab-far-5 were silenced, whereas the proportion of adult nematodes significantly decreased in the nematode population after Ab-far-4 were silenced. (4) Fat storage of nematodes significantly decreased after Ab-far-3, Ab-far-4, and Ab-far-7 were silenced. To our knowledge, this is the first study to demonstrate that Ab-far genes affect sex formation and lipid metabolism in nematodes, which provides valuable data for further study and control of RWTNs.
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影响因子：
4.5
作者：
Coolon JD;Jones KL;Todd TC;Carr BC;Herman MA
通讯作者：
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