Directed Expression of Tracheal Antimicrobial Peptide as a Treatment for Bovine-Associated Staphylococcus Aureus-Induced Mastitis in Mice.
Directed Expression of Tracheal Antimicrobial Peptide as a Treatment for Bovine-Associated Staphylococcus Aureus-Induced Mastitis in Mice.
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DOI：
10.3389/fvets.2021.700930
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发表时间：
2021
影响因子：
3.2
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Zhang Z;Chen D;Lu X;Zhao R;Chen Z;Li M;Xu T;Mao Y;Yang Y;Yang Z
奶牛乳房炎是困扰奶牛业的开始，因为集约化奶牛养殖，这导致了奶牛生产力的降低和成本的上升。抗生素的使用引起了一系列问题，尤其是细菌耐药性的形成。然而，有有限的无抗生素的治疗策略，可以有效地减轻细菌感染的牛乳腺。因此，在这项研究中，我们构建了乳腺组织特异性表达载体携带的抗菌肽的牛源性气管抗菌肽（TAP），并评估它在原代牛乳腺上皮细胞（pBMEC）和小鼠。结果表明，β-乳球蛋白基因（BLG）启动子驱动的载体能有效地指导TAP在pBMEC和小鼠乳腺组织中的表达。此外，显着的抗菌效果，观察在体外和体内实验时，引入该载体牛相关的金黄色葡萄球菌处理的pBMEC和小鼠，分别。本研究表明，乳腺组织特异性表达载体可在体内外导入抗菌肽，为奶牛乳腺炎的治疗提供了新的思路。
Bovine mastitis is perplexing the dairy industry since the initiation of intensive dairy farming, which has caused a reduction in the productivity of cows and an escalation in costs. The use of antibiotics causes a series of problems, especially the formation of bacterial antimicrobial resistance. However, there are limited antibiotic-free therapeutic strategies that can effectively relieve bacterial infection of bovine mammary glands. Hence, in this study, we constructed a mammary gland tissue-specific expression vector carrying the antimicrobial peptide of bovine-derived tracheal antimicrobial peptide (TAP) and evaluated it in both primary bovine mammary epithelial cells (pBMECs) and mice. The results showed that the vector driven by the β-lactoglobulin gene (BLG) promoter could efficiently direct the expression of TAP in pBMECs and the mammary gland tissue of mice. In addition, significant antibacterial effects were observed in both in vitro and in vivo experiments when introducing this vector to bovine-associated Staphylococcus aureus-treated pBMECs and mice, respectively. This study demonstrated that the mammary gland tissue-specific expression vector could be used to introduce antimicrobial peptide both in in vitro and in vivo and will provide a new therapeutic strategy in the treatment of bovine mastitis.
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DOI：
10.1073/pnas.88.9.3952
发表时间：
1991-05-01
影响因子：
11.1
作者：
DIAMOND, G;ZASLOFF, M;BEVINS, CL
通讯作者：
BEVINS, CL
影响因子：
4.9
作者：
BERGHASH, SR;DAVIDSON, JN;DUNNY, GM
通讯作者：
DUNNY, GM
影响因子：
1.7
作者：
Fan, Jianyong;Luo, Yuan;Li, Nie
通讯作者：
Li, Nie
影响因子：
4
作者：
Li, WenHua;Li, Yong;Wang, Qiang
通讯作者：
Wang, Qiang
影响因子：
5.2
作者：
Canovas J;Baldry M;Bojer MS;Andersen PS;Grzeskowiak PK;Stegger M;Damborg P;Olsen CA;Ingmer H
通讯作者：
Ingmer H