Alignment of collagen matrices using magnetic nanowires and magnetic barcode readout using first order reversal curves (FORC) (invited)
Alignment of collagen matrices using magnetic nanowires and magnetic barcode readout using first order reversal curves (FORC) (invited)
复制标题
使用磁性纳米线对齐胶原蛋白基质并使用一阶反转曲线（FORC）读取磁性条形码（特邀）
DOI：
10.1016/j.jmmm.2017.11.035
复制
发表时间：
2018
影响因子：
2.7
通讯作者：
Hubel, Allison
中科院分区：
文献类型：
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作者：
Sharma, Anirudh;DiVito, Michael D.;Shore, Daniel E.;Block, Andrew D.;Pollock, Katie;Solheid, Peter;Feinberg, Joshua M.;Modiano, Jaime;Lam, Cornelius H.;Hubel, Allison
胶原蛋白基质是一种人工组织，在仿生器官或肿瘤以及基础生物学中有应用。解剖器官和组织通常由排列的胶原蛋白组成，在本研究中，将镍磁性纳米线(MNWs)与胶原蛋白交联后，当在均匀磁场中处理时，胶原蛋白基质可以一步双向排列。用差示干涉显微镜(DIC)、扫描电子显微镜(SEM)和偏光透过率对这些基质进行了分析。这种双向排列也被来自血脑屏障(BBB)的染色的蛛网膜细胞所证实。蛛网膜细胞对其细胞外基质(ECM)环境在形态上是敏感的，在本研究中，根据显微镜和透射率的预测，它们被观察到在两个不同的方向上蜘蛛。事实上，植入蛛网膜细胞的MNW-胶原基质有望成为未来人工BBBS的研究对象。已经观察到其他细胞(这里是骨肉瘤)内化mNW，这导致了条形码基质和具有不同特征的细胞的可能性，等待磁读出技术。为此，用一阶逆转曲线(FORC)分析了两个不同MNW种群的混合物，正确地估计了两个种群的相对浓度，当比例为1：23时，误差可以忽略不计，而当比例为1：115时，误差仅为7%。总之，这些研究为人工组织的磁性识别开辟了一条道路，基质和细胞内不同的磁性标记结合在一起，形成了独特的指纹。
Collagen matrices are one form of artificial tissue that has applications in biomimetic organs or tumors, and in fundamental biology. Anatomical organs and tissues are often composed of aligned collagen, and in this study cross-linking nickel magnetic nanowires (MNWs) to collagen allowed a one-step bi-directional alignment of the collagen matrices when processed in a uniform magnetic field. These matrices were analyzed by differential interference contrast (DIC) microscopy, scanning electron microscopy (SEM) and polarized transmittance. The bi-directional alignment was also confirmed by plated, stained arachnoid cells from the blood-brain-barrier (BBB). Arachnoid cells are morphologically sensitive to their extracellular matrix (ECM) environment, and in this study, they were observed to spider out in two distinct directions as predicted by microscopy and transmittance. In fact, MNW-collagen matrices plated with arachnoid-cells are promising for future studies of artificial BBBs. Other cells (here osteosarcoma) have been observed to internalize MNWs, which leads to the possibility of barcoding matrices and cells with distinct signatures, pending a magnetic readout technique. To this aim, mixtures of two different MNW populations were analyzed using first order reversal curves (FORC), and the relative concentrations of the two populations were correctly estimated with negligible error for ratios of 1: 23 and only 7% error for ratios of 1: 115. Together, these studies open a path for magnetic identification of artificial tissues where distinct magnetic labels on matrices and in cells combine for a unique fingerprint.
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影响因子：
3.7
作者：
Wells JW;Evans CH;Scott MC;Rütgen BC;O'Brien TD;Modiano JF;Cvetkovic G;Tepic S
通讯作者：
Tepic S
影响因子：
4.9
作者：
Zhong, Shaoping;Teo, Wee Eong;Yung, Lin Yue Lanry
通讯作者：
Yung, Lin Yue Lanry
影响因子：
56.9
作者：
RUOSLAHTI, E;PIERSCHBACHER, MD
通讯作者：
PIERSCHBACHER, MD
影响因子：
3.3
作者：
Janson, C.;Romanova, L.;Lam, C.
通讯作者：
Lam, C.
影响因子：
38.3
作者：
通讯作者：
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