Radioprotective effect of American ginseng on human lymphocytes at 90 minutes postirradiation: a study of 40 cases.
Radioprotective effect of American ginseng on human lymphocytes at 90 minutes postirradiation: a study of 40 cases.
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DOI：
10.1089/acm.2009.0590
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发表时间：
2010-05
期刊：
影响因子：
--
通讯作者：
Allison RR
中科院分区：
文献类型：
--
作者：
Lee TK;O'Brien KF;Wang W;Johnke RM;Sheng C;Benhabib SM;Wang T;Allison RR
电离辐射（IR）通过增强活性氧（ROS）的形成引发细胞内氧化应激，ROS攻击DNA，导致细胞死亡。随着红外辐射在医学、农业、工业等领域应用的多样化，以及全球恐怖主义威胁的日益严重，国家对辐射防护剂的需求日益迫切。然而，目前正在研究的辐射防护剂的适用性是有限的，由于其固有的毒性。本研究探讨了标准化的北美人参提取物（NAGE，总皂苷含量：11.7%）对照射后90分钟人淋巴细胞DNA损伤的影响。照射（1和2戈伊）后90分钟应用NAGE（250 - 1000 µg ml-1），通过胞质分裂阻滞微核（CBMN）测定评估了从40名健康个体中获得的淋巴细胞的DNA损伤。在用WR-1065（ImM或3 mM）处理的淋巴细胞中也进行了类似的实验。此外，在照射前后，从10个人获得的淋巴细胞测定其总抗氧化能力（TAC）和活性氧（ROS）。NAGE对~（137）Cs诱导的淋巴细胞微核的显著抑制作用具有浓度依赖性。NAGE（750 µg ml-1）在1戈伊照射后使MN产量分别减少50.7%，在2戈伊照射后使MN产量分别减少35.9%;这些结果与WR-1065的结果相当。此外，我们还发现，NAGE降低MN产量和ROS，但增加淋巴细胞中的TAC。我们的研究结果表明，NAGE是一种相对无毒的天然化合物，即使在照射后90分钟应用时，也能在人体淋巴细胞中保持辐射防护潜力。其辐射防护机制之一可能是通过清除自由基和增强细胞内TAC来介导的。
Ionizing radiation (IR) initiates intracellular oxidative stress through enhanced formation of reactive oxygen species (ROS) that attack DNA leading to cell death. As the diversity of IR applied in medicine, agriculture, industry, and the growing threats of global terrorism, the acquisition of radioprotectors is an urgent need for the nation. However, the applicability of radioprotectors currently under investigation is limited due to their inherent toxicity. This study investigated the effect of a standardized North American ginseng extract (NAGE, total ginsenoside content: 11.7%) on DNA damage in human lymphocytes at 90 min post-irradiation. With the application of NAGE (250 – 1000 µg ml−1) at 90 min post-irradiation (1 and 2 Gy), DNA damage in lymphocytes obtained from 40 healthy individuals was evaluated by cytokinesis-block micronucleus (CBMN) assay. Similar experiments were also performed in lymphocytes treated with WR-1065 (1 mM or 3mM). In addition, before and after irradiation, lymphocytes obtained from 10 individuals were measured for their total antioxidant capacity (TAC) and the reactive oxygen species (ROS). The significant effect of NAGE against 137Cs-induced MN in lymphocytes is concentration-dependent. NAGE (750 µg ml−1) reduced MN yield by 50.7% after 1 Gy and 35.9% after 2 Gy exposures, respectively; these results were comparable to that of WR-1065. Further, we also found that NAGE reduces MN yield and ROS but increases TAC in lymphocytes. Our results suggest that NAGE is a relatively non-toxic natural compound that holds radioprotective potential in human lymphocytes even when applied at 90 min post-irradiation. One of the radioprotective mechanisms may be mediated through the scavenging of free radicals and enhancement of the intracellular TAC.
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影响因子：
2.7
作者：
Lemon, J. A.;Rollo, C. D.;Boreham, D. R.
通讯作者：
Boreham, D. R.
影响因子：
3.4
作者：
Pellmar, Terry C;Rockwell, Sara
通讯作者：
Rockwell, Sara
影响因子：
2.9
作者：
Block, Keith I;Mead, Mark N
通讯作者：
Mead, Mark N
DOI：
10.1016/j.mrfmmm.2004.02.018
发表时间：
2004-07-13
影响因子：
2.3
作者：
Moller, P;Loft, S
通讯作者：
Loft, S
DOI：
10.1016/0165-1161(85)90015-9
发表时间：
1985-01-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
FENECH, M;MORLEY, AA
通讯作者：
MORLEY, AA