Profiling and identification of small rDNA-derived RNAs and their potential biological functions.
Profiling and identification of small rDNA-derived RNAs and their potential biological functions.
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小 rDNA 衍生 RNA 的分析和鉴定及其潜在的生物学功能
DOI：
10.1371/journal.pone.0056842
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhai Q
中科院分区：
文献类型：
--
作者：
Wei H;Zhou B;Zhang F;Tu Y;Hu Y;Zhang B;Zhai Q
小的非编码RNA构成了具有不同功能的调节分子的大家族。值得注意的是，一些与rDNA匹配的小的非编码RNA已被鉴定为qiRNA和参与各种生物过程的小的指导RNA。然而，大量的小rDNA衍生的RNA（srRNA）通常被忽视，尚未进行研究。我们利用高通量测序得到的小RNA数据集对srRNA进行了系统的研究，发现srRNA主要以有义方向定位于rRNA编码区。来自免疫沉淀和高通量测序的数据集表明，srRNA与Argonaute（AGO）蛋白共免疫沉淀。此外，小鼠肝脏中的srRNA表达谱受到糖尿病的影响。srRNA在培养细胞中的过表达或抑制表明srRNA参与多种信号通路。本研究对不同物种的总小RNA和AGO蛋白复合物中的srRNA进行了全面的研究，并证实了srRNA与糖尿病相关，参与了一些生物学过程。这些发现为srRNA及其在各种生理和病理过程中的功能提供了新的见解。
Small non-coding RNAs constitute a large family of regulatory molecules with diverse functions. Notably, some small non-coding RNAs matched to rDNA have been identified as qiRNAs and small guide RNAs involved in various biological processes. However, a large number of small rDNA-derived RNAs (srRNAs) are usually neglected and yet to be investigated. We systematically investigated srRNAs using small RNA datasets generated by high-throughput sequencing, and found srRNAs are mainly mapped to rRNA coding regions in sense direction. The datasets from immunoprecipitation and high-throughput sequencing demonstrate that srRNAs are co-immunoprecipitated with Argonaute (AGO) proteins. Furthermore, the srRNA expression profile in mouse liver is affected by diabetes. Overexpression or inhibition of srRNAs in cultured cells shows that srRNAs are involved in various signaling pathways. This study presents a global view of srRNAs in total small RNA and AGO protein complex from different species, and demonstrates that srRNAs are correlated with diabetes, and involved in some biological processes. These findings provide new insights into srRNAs and their functions in various physiological and pathological processes.
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影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
影响因子：
3.7
作者：
Elbarbary RA;Takaku H;Uchiumi N;Tamiya H;Abe M;Takahashi M;Nishida H;Nashimoto M
通讯作者：
Nashimoto M
影响因子：
14.9
作者：
Barrett T;Troup DB;Wilhite SE;Ledoux P;Evangelista C;Kim IF;Tomashevsky M;Marshall KA;Phillippy KH;Sherman PM;Muertter RN;Holko M;Ayanbule O;Yefanov A;Soboleva A
通讯作者：
Soboleva A
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.4
作者：
GONZALEZ, IL;SYLVESTER, JE
通讯作者：
SYLVESTER, JE