Molecular effects of soy phytoalexin glyceollins in human prostate cancer cells LNCaP.
Molecular effects of soy phytoalexin glyceollins in human prostate cancer cells LNCaP.
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DOI：
10.1002/mc.20532
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发表时间：
2009-09
影响因子：
4.6
通讯作者：
Wang, Thomas T. Y.
中科院分区：
文献类型：
--
作者：
Payton-Stewart, Florastina;Schoene, Norberta W.;Kim, Young S.;Burow, Matthew E.;Cleveland, Thomas E.;Boue, Stephen M.;Wang, Thomas T. Y.
甘油三酯是从大豆中提取的植物保健素，已被认为是预防癌症的候选化合物。甘氨酸甘油酯对前列腺癌的作用尚不清楚。本研究检测了大豆植物保健素(Glyceollins)对人前列腺癌LNCaP细胞的分子作用，以进一步阐明其预防前列腺癌的潜在作用。我们发现，大豆异黄酮染料木素对LNCaP细胞生长的抑制作用与大豆异黄酮染料木素相似。甘油三酯的生长抑制作用可能是由于抑制了G1/S的进程，并与上调细胞周期蛋白依赖性激酶抑制因子1A和B的基因和蛋白水平有关。相比之下，金雀异黄素只上调细胞周期蛋白依赖的激酶抑制因子1A。此外，甘氨酸甘油处理导致雄激素反应基因的mRNA水平下调。与金雀异黄素不同，甘油三酯对雄激素反应基因的这种影响似乎是通过调节雌激素而不是雄激素介导的途径来实现的。因此，甘油三酯对LNCaP细胞具有多种作用，可能被认为是防癌的，其作用机制似乎与其他大豆提取的植物化学物质不同。
Glyceollins are soy-derived phytoalexins that have been proposed to be candidate cancer preventive compounds. The effect of the glyceollins on prostate cancer is unknown. The present study examined the molecular effects of soy phytoalexin, glyceollins, on human prostate cancer cell LNCaP to further elucidate its potential effects on prostate cancer prevention. We found that the glyceollins inhibited LNCaP cell growth similar to that of the soy isoflavone genistein. The growth inhibitory effects of the glyceollins appeared to be due to an inhibition of G1/S progression and correlated with an up-regulation of cyclin-dependent kinase inhibitor 1 A and B mRNA and protein levels. By contrast, genistein only up-regulates cyclin-dependent kinase inhibitor 1A. In addition, glyceollin treatments led to down-regulated mRNA levels for androgen responsive genes. In contrast to genistein, this effect of glyceollins on androgen responsive genes appeared to be mediated through modulation of an estrogen- but not androgen-mediated pathway. Hence, the glyceollins exerted multiple effects on LNCaP cells that may be considered cancer preventive and the mechanisms of action appeared to be different from other soy-derived phytochemicals.
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影响因子：
4.2
作者：
Lamartiniere, CA;Cotroneo, MS;Elgavish, A
通讯作者：
Elgavish, A
影响因子：
4.7
作者：
El Touny, Lara. H.;Banerjee, Partha P.
通讯作者：
Banerjee, Partha P.
影响因子：
5.8
作者：
Burow, ME;Boue, SM;McLachlan, JA
通讯作者：
McLachlan, JA
影响因子：
4.8
作者：
CASTAGNETTA, LA;MICELI, MD;CARRUBA, G
通讯作者：
CARRUBA, G
影响因子：
4.7
作者：
Hong, CB;Kim, HA;Bjeldanes, LF
通讯作者：
Bjeldanes, LF