PLK1-mediated S369 phosphorylation of RIPK3 during G2 and M phases enables its ripoptosome incorporation and activity.
PLK1-mediated S369 phosphorylation of RIPK3 during G2 and M phases enables its ripoptosome incorporation and activity.
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DOI：
10.1016/j.isci.2021.102320
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发表时间：
2021-04-23
期刊：
影响因子：
5.8
通讯作者：
Liu B
中科院分区：
文献类型：
--
作者：
Gupta K;Liu B
Receptor-interacting protein kinase 3 executes a form of regulated necrosis called necroptosis. Upon induction of an altered conformation by chemical inhibitors or via mutations in its kinase site, RIPK3 associates with a multiprotein complex called the ripoptosome—a signaling platform containing FADD, RIPK1, caspase 8, and cFLIP—and becomes decisive in the execution of apoptosis. Surprisingly, in contexts not completely understood, the ripoptosome itself cleaves RIPK3, highlighting an apparent conundrum on how RIPK3 fulfills its role via the complex responsible for its own degradation. Recently, ripoptosome assembly was found to occur in mitosis where we found elevated RIPK3 levels. We now report that PLK1 directly associates with RIPK3 and phosphorylates it at S369 as cells enter mitosis. G2/M phase RIPK3 has pro-apoptotic activity but upon release from ripoptosome, can trigger necroptosis. Taken together, phosphorylation of RIPK3 at S369 prevents its ripoptosome-mediated cleavage thereby retaining its pro-death activity during mitosis. Inhibiting RIPK3, a necroptotic kinase, triggers apoptosis via the ripoptosome Ripoptosome normally cleaves RIPK3 and is assembled in mitosis pS369 in G2/M phases prevents RIPK3 proteolysis via ripoptosome Phosphorylation of S369 by PLK1 enables cell death plasticity in G2/M phases Biological Sciences; Cell Biology; Functional Aspects of Cell Biology
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DOI：
10.1042/bj20130860
发表时间：
2013-12-15
期刊：
The Biochemical journal
影响因子：
--
作者：
McQuade T;Cho Y;Chan FK
通讯作者：
Chan FK
DOI：
10.1083/jcb.200309035
发表时间：
2004-01-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lindon C;Pines J
通讯作者：
Pines J
影响因子：
64.8
作者：
Oberst, Andrew;Dillon, Christopher P.;Weinlich, Ricardo;McCormick, Laura L.;Fitzgerald, Patrick;Pop, Cristina;Hakem, Razq;Salvesen, Guy S.;Green, Douglas R.
通讯作者：
Green, Douglas R.
影响因子：
16
作者：
Mandal P;Berger SB;Pillay S;Moriwaki K;Huang C;Guo H;Lich JD;Finger J;Kasparcova V;Votta B;Ouellette M;King BW;Wisnoski D;Lakdawala AS;DeMartino MP;Casillas LN;Haile PA;Sehon CA;Marquis RW;Upton J;Daley-Bauer LP;Roback L;Ramia N;Dovey CM;Carette JE;Chan FK;Bertin J;Gough PJ;Mocarski ES;Kaiser WJ
通讯作者：
Kaiser WJ
影响因子：
16
作者：
Liccardi, Gianmaria;Garcia, Laura Ramos;Meier, Pascal
通讯作者：
Meier, Pascal