RNA sequencing and transcriptomal analysis of human monocyte to macrophage differentiation.
RNA sequencing and transcriptomal analysis of human monocyte to macrophage differentiation.
复制标题
DOI：
10.1016/j.gene.2013.02.015
复制
发表时间：
2013-05-01
期刊：
影响因子：
3.5
通讯作者：
Xiong S
中科院分区：
文献类型：
--
作者：
Dong C;Zhao G;Zhong M;Yue Y;Wu L;Xiong S
单核细胞可以在体内分化为巨噬细胞，这些细胞在先天性和适应性免疫应答中起重要作用。为了揭示单核细胞向巨噬细胞分化过程中发生的整体基因转录变化，我们在人原代单核细胞和单核细胞衍生的巨噬细胞中进行了全基因组RNA测序和分析。我们发现，与单核细胞相比，巨噬细胞中有1208个基因（差异>两倍）差异表达，包括800个上调和408个下调基因。基因本体、通路和蛋白质-蛋白质相互作用分析表明，上调的基因与巨噬细胞的吞噬功能、代谢过程和细胞周期有关。大多数下调的基因包括参与炎症反应和运动的基因。转录调控因子FOXO 1、RUNX 3、NF-κB1和C/EBP δ等基因在单核细胞中高度表达，似乎具有显著的转录抑制作用，导致轻微的代谢活性。我们使用RNA测序在人单核细胞和单核细胞衍生的巨噬细胞之间进行的转录组比较揭示了与分化过程相关的新分子和途径。这些分子和途径可能代表参与这些重要免疫细胞的病理生理学的候选靶标。
Monocytes can be differentiated into macrophages in vivo and these cells play an important role in innate and adaptive immune responses. To reveal the global gene transcription change that occurs during monocyte to macrophage differentiation, we performed genome-wide RNA sequencing and analyses in human primary monocytes and monocyte-derived macrophages. We show that 1208 genes (with >twofold differences) were differentially expressed in macrophages compared with monocytes, including 800 upregulated and 408 downregulated genes. Gene ontology, pathway, and protein–protein interaction analyses indicated that the upregulated genes were related to macrophage functions in phagocytosis, metabolic processes, and cell cycle. The majority of downregulated genes comprised genes involved in the inflammatory response and locomotion. Genes encoding transcription regulatory factors, such as FOXO1, RUNX3, NF-κB1, and C/EBP δ, were highly expressed in monocytes and appeared to function in significant transcriptional repression, resulting in slight metabolic activity. Our transcriptome comparison between human monocytes and monocyte-derived macrophages using RNA sequencing revealed novel molecules and pathways associated with the differentiation process. These molecules and pathways may represent candidate targets involved in the pathophysiology of these important immune cells.
登录
查看更多内容
影响因子：
20.3
作者：
Hashimoto, S;Suzuki, T;Matsushima, K
通讯作者：
Matsushima, K
影响因子：
64.8
作者：
Goldstone, David C.;Ennis-Adeniran, Valerie;Webb, Michelle
通讯作者：
Webb, Michelle
影响因子：
4.8
作者：
Kodvawala, A;Ghering, AB;Hui, DY
通讯作者：
Hui, DY
影响因子：
5.4
作者：
Dong, Chunsheng;Janas, Alicia M.;Wu, Li
通讯作者：
Wu, Li
DOI：
10.1016/s0167-4889(02)00398-1
发表时间：
2003-02-17
影响因子：
5.1
作者：
Hansson, M;Ellis, E;Babiker, A
通讯作者：
Babiker, A