The gene ygdP, associated with the invasiveness of Escherichia coli K1, designates a Nudix hydrolase, Orf176, active on adenosine (5')-pentaphospho-(5')-adenosine (Ap5A).
The gene ygdP, associated with the invasiveness of Escherichia coli K1, designates a Nudix hydrolase, Orf176, active on adenosine (5')-pentaphospho-(5')-adenosine (Ap5A).
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基因 ygdP 与大肠杆菌 K1 的侵袭性相关，指定 Nudix 水解酶 Orf176，对腺苷 (5)-五磷酸-(5)-腺苷 (Ap5A) 具有活性。
DOI：
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发表时间：
2001
影响因子：
4.8
通讯作者：
Jianying Shen
中科院分区：
文献类型：
--
作者：
M. Bessman;J. Walsh;Christopher A. Dunn;Jyothishmathi Swaminathan;John E. Weldon;Jianying Shen
Ygdp，一个与大肠杆菌K1侵袭脑微血管内皮细胞相关的基因(Badger，J.L.，Wass，C.A.和Kim，K.S.(2000)Mol.微生物。36,174-182)是引起新生儿脑膜炎的主要革兰氏阴性菌，已被克隆并在大肠杆菌中表达。该蛋白YgdP被纯化到接近均一的水平，并被鉴定为二核苷寡磷酸焦磷酸酶Nudex水解酶亚家族的成员。它催化二腺苷四磷酸、五磷酸和六磷酸的水解，优先于二腺苷五磷酸，从中形成三磷酸腺苷和二磷酸腺苷。这种酶对二价金属阳离子的要求是可以与镁、锌或锰离子结合的，并且像大多数Nudex水解酶一样，碱性pH最适在8.5到9之间。这是第二次鉴定出与人类病原体侵袭性相关的基因，它是二核苷寡磷焦磷酸酶Nudex水解酶亚家族的成员，对其他入侵细菌的同源蛋白的检测表明，这可能是细胞入侵的共同特征。
ygdP, a gene associated with the invasion of brain microvascular endothelial cells by Escherichia coli K1 (Badger, J. L., Wass, C. A., and Kim, K. S. (2000) Mol. Microbiol. 36, 174-182), the primary Gram-negative bacterium causing meningitis in newborns, has been cloned and expressed in E. coli. The protein, YgdP, was purified to near homogeneity and identified as a member of the Nudix hydrolase subfamily of dinucleoside oligophosphate pyrophosphatases. It catalyzes the hydrolysis of diadenosine tetra-, penta-, and hexa-phosphates with a preference for diadenosine penta-phosphate, from which it forms ATP and ADP. The enzyme has a requirement for a divalent metal cation that can be met with Mg2+, Zn2+, or Mn2+ and, like most of the Nudix hydrolases, has an alkaline pH optimum between 8.5 and 9. This is the second identification of a gene associated with the invasiveness of a human pathogen as a member of the Nudix hydrolase subfamily of dinucleoside oligophosphate pyrophosphatases, and an examination of homologous proteins in other invasive bacteria suggests that this may be a common feature of cellular invasion.
DOI：
10.1073/pnas.80.24.7496
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LEE, PC;BOCHNER, BR;AMES, BN
通讯作者：
AMES, BN
DOI：
10.1006/bbrc.1999.0354
发表时间：
1999-03-24
影响因子：
3.1
作者：
Cartwright, JL;Britton, P;McLennan, AG
通讯作者：
McLennan, AG