CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the α6/β1 integrin and the neural adhesion molecule L1
CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the α6/β1 integrin and the neural adhesion molecule L1
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小鼠大脑的 CD9 参与体外神经突生长和细胞迁移，并与 α6/β1 整合素和神经粘附分子 L1 相关
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
M. Schachner
中科院分区：
文献类型：
--
作者：
C. Schmidt;V. Künemund;E. Wintergerst;B. Schmitz;M. Schachner
我们在这里描述了一种新的单克隆抗体（mab INTRODUCTION H6），它可以识别CD9，这是一种完整的细胞表面成分，以前在造血细胞谱系中被描述过，并参与血小板聚集。H6单抗作用于未成熟小鼠星形胶质细胞膜，并与成年小鼠脑膜洗涤剂提取物中25-27 kD的蛋白反应。N端氨基酸序列分析显示96%与小鼠肾CD9同源。CD9定位于小鼠中枢和外周神经系统：在11天龄小鼠胚胎的脊髓中，CD9在底板和顶板中强烈表达。在成年小鼠坐骨神经中，髓鞘具有高度的cd9免疫反应。Mab H6与培养的胶质细胞和神经元细胞表面反应，抑制小脑微外植体的神经元细胞体迁移、神经突束化和星形细胞突起的生长。mab H6存在于底物包被的层粘连蛋白上，而不存在于底物包被的聚L赖氨酸上，分离的小小脑神经元的神经突生长增加。添加单抗H6引起底物包被层粘连蛋白的细胞内Ca2+浓度增加。培养的小鼠神经母细胞瘤N2A细胞的CD9免疫沉淀含有α6/β1整合素。此外，使用mab H6柱从成年小鼠脑中纯化的CD9免疫亲和制剂含有神经粘附分子L1，但不含其他神经粘附分子。CD9可与L1结合，但不能与NCAM和MAG结合，α6/β1整合素和L1均可被诱导与CD9共同分布在培养的N2A神经母细胞瘤细胞表面。综合观察结果表明，CD9可与L1和α6/β1整合素结合，影响体外神经细胞相互作用。©1996 Wiley‐Liss, Inc。
We describe here a novel monoclonal antibody (mab INTRODUCTION H6) which recognizes CD9, an integral cell surface constituent previously described in cells of the hematopoietic lineage and involved in the aggregation of platelets. Mab H6 was raised against membranes of immature mouse astrocytes and reacted with a protein of 25–27 kD in detergent extracts of adult mouse brain membranes. Sequence analysis of the N‐terminal amino acids revealed an identity of 96% with CD9 from mouse kidney. CD9 was localized in the central and peripheral mouse nervous systems: in the spinal cord of 11‐day‐old mouse embryos, CD9 was strongly expressed in the floor and roof plates. In the adult mouse sciatic nerve, myelin sheaths were highly CD9immunoreactive. Mab H6 reacted with the cell surfaces of both glial cells and neurons in culture and inhibited migration of neuronal cell bodies, neurite fasciculation and outgrowth of astrocytic processes from cerebellar microexplants. Neurite outgrowth from isolated small cerebellar neurons was increased in the presence of mab H6 on substrate‐coated laminin, but not on substrate‐coated poly‐L‐lysine. Addition of mab H6 elicited an increase in intracellular Ca2+ concentration in these cells on substrate‐coated laminin. Immunoprecipitates of CD9 from cultured mouse neuroblastoma N2A cells contained the α6/β1 integrin. Moreover, preparations of CD9 immunoaffinity‐purified from adult mouse brain using a mab H6 column contained the neural adhesion molecule L1, but not other neural adhesion molecules. CD9 bound to L1, but not to NCAM or MAG. Both the α6/β1 integrin and L1 could be induced to coredistribute with CD9 on the surface of cultured neuroblastoma N2A cells. The combined observations suggest that CD9 can associate with L1 and α6/β1 integrin to influence neural cell interactions in vitro. © 1996 Wiley‐Liss, Inc.
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DOI：
10.1016/0167-4889(92)90144-z
发表时间：
1992
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Kroll,MH;Mendelsohn,ME;Miller,JL;Ballen,KK;Hrbolich,JK;Schafer,AI
通讯作者：
Schafer,AI
血小板p24/CD9的cDNA克隆和表达。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lanza,F;Wolf,D;Fox,CF;Kieffer,N;Seyer,JM;Fried,VA;Coughlin,SR;Phillips,DR;Jennings,LK
通讯作者：
Jennings,LK
抗人血小板 p24/CD9 单克隆抗体介导的人血小板活化。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jennings,LK;Fox,CF;Kouns,WC;McKay,CP;Ballou,LR;Schultz,HE
通讯作者：
Schultz,HE
影响因子：
56.9
作者：
OTOOLE, TE;MANDELMAN, D;GINSBERG, MH
通讯作者：
GINSBERG, MH
来自眼睫状上皮细胞的牛 CD9 抗原的分子克隆。
影响因子：
2.7
作者：
Martín-Alonso,JM;Hernando,N;Ghosh,S;Coca-Prados,M
通讯作者：
Coca-Prados,M