Plant regeneration of the arsenic hyperaccumulator Pteris vittata L. from spores and identification of its tolerance and accumulation of arsenic and copper
Plant regeneration of the arsenic hyperaccumulator Pteris vittata L. from spores and identification of its tolerance and accumulation of arsenic and copper
复制标题
砷超富集植物 Pteris vittata L. 孢子再生植株及其对砷和铜的耐受和积累鉴定
DOI：
10.1007/s11738-007-0114-6
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发表时间：
2008-03
影响因子：
2.6
通讯作者：
中科院分区：
文献类型：
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作者：
建立了以蜈蚣蕨（Pteris vittata）孢子为外植体的离体植株再生体系，研究了其对砷（As）和铜（Cu）的耐受性、配子体和愈伤组织的积累。用0.5 mg l−1 6-苄基氨基嘌呤（6-BA）+ 0.5 mg l−1赤霉素酸（GA）处理的配子体外植体的愈伤组织形成频率最高（100%）。此外，在补充有0.5 mg l−1 6-BA、0.5-1.0 mg l−1 GA和另外的300 mg l−1乳清蛋白水解物（LH）的MS培养基上4周，从源自配子体外植体的愈伤组织分化孢子体。以1/2 MS + 1.0 mg l−1 GA + 0.5 mg l−1 6-BA + 300 mg l−1 LH为最佳组合，孢子体形成率为75 ± 10%。每个配子体愈伤组织可分化出3-4个孢子体。配子体和愈伤组织的体外生长在培养基中含有低于0.5 mM的Na_3AsO_4加速，但这种生长与2 mM Na_3AsO_4抑制。随着培养基中Na 3AsO 4浓度从0增加到2 mM，配子体和愈伤组织中的As积累量增加，分别达到763.3和315.4 mg kg−1。将配子体和愈伤组织移植到添加不同浓度CuSO 4的培养基中，与Na 3AsO 4中的情况相似，当配子体在含有3 mM CuSO 4的培养基中继代培养时，配子体中的Cu积累可达到7，940 mg kg−1。这些结果表明，该高效繁殖体系可以作为鉴定其他重金属耐性和积累的有效和快速的手段。此外，愈伤组织的再生能力为该蕨类植物的遗传转化提供了可能。
An in vitro plant regeneration system was established from the spores of Pteris vittata and identification of its tolerance, and accumulation of gametophytes and callous, to arsenic (As) and copper (Cu) was investigated. The highest frequency (100%) of callus formation was achieved from gametophyte explants treated with 0.5 mg l−1 6-benzylaminopurine (6-BA) + 0.5 mg l−1 gibberellin acid (GA). Furthermore, sporophytes were differentiated from the callus tissue derived from gametophyte explants on MS medium supplemented with 0.5 mg l−1 6-BA, 0.5–1.0 mg l−1 GA and additional 300 mg l−1 lactalbumin hydrolysate (LH) for 4 weeks. The optimum combination of ½ MS + 1.0 mg l−1 GA + 0.5 mg l−1 6-BA + 300 mg l−1 LH promoted sporophyte formation on 75 ± 10% of the callus. Every callus derived from gametophyte explants could achieve 3–4 sporophytes. The in vitro growth of gametophyte and callus was accelerated in the medium containing Na3AsO4 lower than 0.5 mM, but this growth was inhibited with 2 mM Na3AsO4. And with the increase of Na3AsO4 in the culture medium from 0 to 2 mM, the As accumulation in gametophytes and callus increased and achieved a level of 763.3 and 315.4 mg kg−1, respectively. Gametophytes and calluses transplanted to culture medium, supplemented with different concentrations of CuSO4, are similar to those in Na3AsO4, and the Cu accumulation in gametophytes could achieve 7,940 mg kg−1 when gametophytes were subcultured in medium containing 3 mM CuSO4. These results suggested that the high efficiency propagation system could be a useful and rapid means to identify other heavy metal tolerance and accumulation. Further, the regeneration ability of callus made it possible for genetic transformation of this fern.
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影响因子：
3.7
作者：
A. Salido;Kelly L Hasty;Jae-Min Lim;D. Butcher
通讯作者：
A. Salido;Kelly L Hasty;Jae-Min Lim;D. Butcher
DOI：
--
发表时间：
2000
期刊：
--
影响因子：
--
作者：
D. Salt;U. Kraemer
通讯作者：
D. Salt;U. Kraemer
影响因子：
3
作者：
Hassanein, A;Dorion, N
通讯作者：
Dorion, N
DOI：
10.1007/bf02858324
发表时间：
1987-10
期刊：
The Botanical Review
影响因子：
--
作者：
E. Sheffield;P. Bell
通讯作者：
E. Sheffield;P. Bell
影响因子：
4.3
作者：
H. Fernández;A. Bertrand;R. Sánchez-Tamés
通讯作者：
H. Fernández;A. Bertrand;R. Sánchez-Tamés