Global Analysis of WOX Transcription Factor Gene Family in Brassica napus Reveals Their Stress- and Hormone-Responsive Patterns.
Global Analysis of WOX Transcription Factor Gene Family in Brassica napus Reveals Their Stress- and Hormone-Responsive Patterns.
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甘蓝型油菜 WOX 转录因子基因家族的整体分析揭示了它们的应激和激素响应模式
DOI：
10.3390/ijms19113470
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发表时间：
2018-11-05
影响因子：
5.6
通讯作者：
Du H
中科院分区：
文献类型：
--
作者：
Wang MM;Liu MM;Ran F;Guo PC;Ke YZ;Wu YW;Wen J;Li PF;Li JN;Du H
植物特异性wuschell相关同源盒（WOX）转录因子家族对植物生长发育具有重要意义，但在油料作物中研究较少。我们从甘蓝型油菜（Brassica napus, BnWOXs）中鉴定并鉴定了58个WOX基因，根据基因结构和保守基序将其划分为3大支系和9个亚支系。共线性分析表明，大多数BnWOXs是异源多倍体和片段重复事件的产物。基因结构分析表明，每个亚枝中的内含子/外显子和蛋白基序都是保守的，RNA测序显示BnWOXs在不同发育阶段的主要组织和/或器官中具有狭窄的表达谱。每个分支的表达模式高度保守，与油菜和甘蓝的姐妹和同源对的表达模式相似。实时定量聚合酶链反应显示，WOX4亚枝成员在非生物胁迫和激素胁迫下被诱导到幼苗根系中，这表明它们在根系发育和非生物胁迫响应中发挥了重要作用。预计有463种蛋白与BnWOXs相互作用，包括分生组织中调节干细胞稳态的肽。该研究为油菜WOX基因家族的进化和表达提供了新的思路，为今后的基因功能研究奠定了基础。
The plant-specific WUSCHEL-related homeobox (WOX) transcription factor gene family is important for plant growth and development but little studied in oil crops. We identified and characterized 58 putative WOX genes in Brassica napus (BnWOXs), which were divided into three major clades and nine subclades based on the gene structure and conserved motifs. Collinearity analysis revealed that most BnWOXs were the products of allopolyploidization and segmental duplication events. Gene structure analysis indicated that introns/exons and protein motifs were conserved in each subclade and RNA sequencing revealed that BnWOXs had narrow expression profiles in major tissues and/or organs across different developmental stages. The expression pattern of each clade was highly conserved and similar to that of the sister and orthologous pairs from Brassica rapa and Brassica oleracea. Quantitative real-time polymerase chain reaction showed that members of the WOX4 subclade were induced in seedling roots by abiotic and hormone stresses, indicating their contribution to root development and abiotic stress responses. 463 proteins were predicted to interact with BnWOXs, including peptides regulating stem cell homeostasis in meristems. This study provides insights into the evolution and expression of the WOX gene family in B. napus and will be useful in future gene function research.
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影响因子：
56.9
作者：
Fletcher, LC;Brand, U;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
影响因子：
14.8
作者：
Chou, Kuo-Chen;Shen, Hong-Bin
通讯作者：
Shen, Hong-Bin
影响因子：
1.2
作者：
He Z;Cheng F;Li Y;Wang X;Parkin IA;Chalhoub B;Liu S;Bancroft I
通讯作者：
Bancroft I
影响因子：
27.5
作者：
Dolzblasz, Alicja;Nardmann, Judith;Laux, Thomas
通讯作者：
Laux, Thomas
影响因子：
11.6
作者：
Ikeda, Miho;Mitsuda, Nobutaka;Ohme-Takagi, Masaru
通讯作者：
Ohme-Takagi, Masaru