CD4-independent infection by HIV-2 (ROD/B): use of the 7-transmembrane receptors CXCR-4, CCR-3, and V28 for entry.
CD4-independent infection by HIV-2 (ROD/B): use of the 7-transmembrane receptors CXCR-4, CCR-3, and V28 for entry.
复制标题
HIV-2 的 CD4 依赖性感染 (ROD/B)：使用 7 次跨膜受体 CXCR-4、CCR-3 和 V28 进入。
作者：
J. Reeves;Á. McKnight;S. Potempa;G. Simmons;P. Gray;C. Power;T. Wells;R. Weiss;S. Talbot
我们测定了多种7 tm趋化因子受体（CCR-2 B、CCR-3、CCR-4、CCR-5、CXCR-1、CXCR-4）和两种孤儿7 tm受体（V28和EBI.1）允许CD 4阴性猫肾CCC细胞被HIV-2株ROD/A和ROD/B感染的能力。我们发现ROD/B能够利用CCC细胞中瞬时表达的CXCR-4，并且在sCD 4存在下，ROD/A的感染增强了15倍。当在sCD 4存在下培养时，当用趋化因子受体CCR-3或孤儿7 tm受体V2 B瞬时转染时，猫CCC细胞也变得允许ROD/B和ROD/A进入。800 ng/ml嗜酸性粒细胞趋化因子（CCR-3的天然配体）可以阻止ROD/A进入表达CCR-3的CCC细胞。
We have assayed a variety of 7tm chemokine receptors (CCR-2b, CCR-3, CCR-4, CCR-5, CXCR-1, CXCR-4) and two orphan 7tm receptors (V28 and EBI.1) for their ability to allow infection of CD4-negative feline kidney CCC cells by the HIV-2 strains ROD/A and ROD/B. We found that ROD/B was able to use CXCR-4 transiently expressed in CCC cells, and infection by ROD/A was enhanced 15-fold in the presence of sCD4. Feline CCC cells also became permissive to ROD/B and ROD/A entry when transiently transfected with the chemokine receptor CCR-3 or the orphan 7tm receptor V2B, when cultured in the presence of sCD4. Entry of ROD/A into CCC cells expressing CCR-3 could be blocked by 800 ng/ml eotaxin, the natural ligand for CCR-3.
DOI：
10.1073/pnas.83.18.7089
发表时间：
1986-09-01
影响因子：
11.1
作者：
WILEY, CA;SCHRIER, RD;OLDSTONE, MBA
通讯作者：
OLDSTONE, MBA