Water deprivation increases the expression of pituitary adenylate cyclase-activating polypeptide gene in the rat subfornical organ.
Water deprivation increases the expression of pituitary adenylate cyclase-activating polypeptide gene in the rat subfornical organ.
复制标题
缺水会增加大鼠穹窿下器官中垂体腺苷酸环化酶激活多肽基因的表达。
DOI：
10.1210/endo.138.10.5427
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发表时间：
1997
期刊：
影响因子：
4.8
通讯作者：
H. Yamashita
中科院分区：
文献类型：
--
作者：
M. Nomura;Y. Ueta;P. J. Larsen;J. Hannibal;R. Serino;N. Kabashima;I. Shibuya;H. Yamashita
采用免疫组织化学和原位杂交组织化学相结合的方法，在大鼠穹窿下器官（SFO）中检查了缺水对垂体腺苷酸环化酶激活多肽（PACAP）表达的影响。在水合条件下，在 SFO 中观察到 PACAP 免疫反应性 (PACAP-IR) 和 PACAP 基因的表达。与水合充足的动物相比，24 小时和 48 小时的缺水导致 SFO 中 PACAP 基因转录物显着增加。此外，48 小时的缺水导致 PACAP-IR 增加。 PACAP-IR 的这种增加在神经纤维和细胞体中都得到了证实。在 SFO 周围部分，PACAP-IR 细胞体的定位与 PACAP 信使 RNA 合成细胞体之间存在高度相关性。这些结果表明，SFO 中的 PACAP 可能在与缺水后体液平衡调节相关的体液和神经变化中发挥作用。
The effect of water deprivation on the expression of pituitary adenylate cyclase-activating polypeptide (PACAP) was examined in the rat subfornical organ (SFO), using a combination of immunohistochemistry and in situ hybridization histochemistry. In the euhydrated condition, PACAP-immunoreactivity (PACAP-IR) and the expression of PACAP gene was observed in the SFO. Water deprivation for 24 h and 48 h caused a significant increase in PACAP gene transcripts in the SFO, compared with euhydrated animals. Additionally, water deprivation for 48 h caused an increase in PACAP-IR. This increase of PACAP-IR was demonstrated in both nerve fibers and cell bodies. High correlation was found between the localization of PACAP-IR cell bodies and PACAP messenger RNA synthesizing cell bodies in the peripheral part of the SFO. These results suggest that PACAP in the SFO may play a role in the humoral and neural changes associated with the regulation of body fluid balance after water deprivation.
DOI：
10.1016/0006-291x(89)91757-9
发表时间：
1989-10-16
影响因子：
3.1
作者：
MIYATA, A;ARIMURA, A;COY, DH
通讯作者：
COY, DH