Gene transcription studies of Candida albicans following infection of HEp2 epithelial cells.
Gene transcription studies of Candida albicans following infection of HEp2 epithelial cells.
复制标题
HEp2 上皮细胞感染后白色念珠菌的基因转录研究。
DOI：
10.1080/13693780500434701
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发表时间：
2006
期刊：
影响因子：
2.9
通讯作者：
E. Segal
中科院分区：
文献类型：
--
作者：
H. Sandovsky;N. Chauhan;R. Calderone;E. Segal
先前我们观察到，白色念珠菌感染HEp2上皮细胞导致HEp2细胞肌动蛋白重排以及膜褶皱和运动性减少，白色念珠菌培养的上清（念珠菌代谢物）引起同样的变化。在这项研究中，我们使用微阵列分析来确定感染HEp2细胞后白色念珠菌基因转录的变化，与没有HEp2细胞的对照培养物相比。我们观察到201个基因在HEp2细胞孵育3小时后表达量至少增加2倍，87个基因表达量下调。上调的基因包括ALS2和ALS5，这两个基因都编码为白色念珠菌提供粘附功能的蛋白质。为了证实ALS转录的变化，我们每隔1小时用RT-PCR检测ALS1-9，共检测4小时。感染1小时后，与单独生长的白色念珠菌相比，一些ALS基因上调。在2-4小时，在感染和对照培养中观察到大多数ALS基因的增加。ALS7转录仅在3-4小时观察到，但在感染和对照培养中转录相似。通过RT-PCR，与微阵列数据相似，ALS2和5在感染细胞中3小时显著增加。我们的研究结果表明，白色念珠菌粘附HEp2细胞后，基因转录包括编码已知宿主识别粘附素家族成员的基因上调，这可能是成功定植和入侵有机体的关键。
Previously we observed that infection of HEp2 epithelial cells with Candida albicans results in HEp2 cell actin rearrangement as well as reduced membrane ruffling and motility and that supernatants of a C. albicans culture (Candida metabolite) caused the same changes. In this study, we used microarray analysis to determine changes in gene transcription of C. albicans following infection of HEp2 cells compared to control cultures grown in the absence of HEp2 cells. We observed 201 genes whose regulation was increased at least 2-fold following a 3 h incubation with HEp2 cells as well as 87 genes that are down-regulated. Among the up-regulated genes were ALS2 and ALS5 both of which encode proteins that provide an adherence function for C. albicans. To confirm the changes in ALS transcription, we measured by RT-PCR ALS1-9 at 1 h intervals for a total of 4 h. After 1 h of infection, several of the ALS genes were up-regulated compared to C. albicans grown alone. At 2-4 h, an increase in most of the ALS genes was observed in both infected and control cultures. ALS7 transcription was observed only at 3-4 h, but transcription was similar in both infected and control cultures. By RT-PCR, ALS2 and 5, similar to the microarray data, were significantly increased in infected cells at 3 h. Our results show that gene transcription following the adherence of C. albicans to HEp2 cells includes the up-regulation of genes encoding members of a family of known host recognition adhesins that may be critical to successful colonization and invasion of the organism.
影响因子：
3
作者：
Chen, SHM;Stins, MF;Jong, AY
通讯作者：
Jong, AY
非白色念珠菌凝集素样序列基因的表征和 ALS 家族的系统发育分析。
影响因子：
3.3
作者：
Hoyer,LL;Fundyga,R;Hecht,JE;Kapteyn,JC;Klis,FM;Arnold,J
通讯作者：
Arnold,J