The mitochondrial pathway is involved in American ginseng-induced apoptosis of SW-480 colon cancer cells.
The mitochondrial pathway is involved in American ginseng-induced apoptosis of SW-480 colon cancer cells.
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DOI：
10.3892/or_00000259
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发表时间：
2009-03
期刊：
影响因子：
4.2
通讯作者：
Yuan CS
中科院分区：
文献类型：
--
作者：
Wang CZ;Li XL;Wang QF;Mehendale SR;Fishbein AB;Han AH;Sun S;Yuan CS
许多有效的抗癌药物已经从植物来源开发，在草药中仍然有大量未开发的资源。在这项研究中，我们评估了西洋参提取物的化学成分，西洋参提取物中的皂苷对SW-480人结直肠癌细胞的抗增殖作用，以及它们的凋亡机制。将西洋参根在120°C下蒸2或4小时。使用HPLC测定未蒸煮和蒸煮的提取物中的代表性皂苷。MTS法检测人参皂苷Rb 1、Rg 3和Rh 2对SW-480细胞增殖的抑制作用。Annexin V/PI双染流式细胞仪检测蒸4 h提取物对SW-480细胞凋亡的影响。使用实时定量PCR阵列分析确定84个肿瘤相关基因的表达，包括TNF、线粒体和p53通路。用FC-1染色后分析线粒体膜电位（Δ m）。西洋参清蒸处理可显著提高人参中Rg 3和Rh 2的含量及抗增殖活性。定量PCR芯片分析结果表明，线粒体途径中的多个基因参与了西洋参诱导SW-480细胞的凋亡，细胞功能分析结果验证了表达谱的正确性。线粒体途径可能在西洋参介导的癌细胞凋亡中起关键作用。
Numerous effective anticancer drugs have been developed from botanical sources, and there remains a significant untapped resource in herbal medicines. In this study, we evaluated the chemical composition of extracts from American ginseng after steaming, the antiproliferative effects of the ginsenosides in the extracts on SW-480 human colorectal cancer cells, and their apoptotic mechanisms. American ginseng roots were steamed at 120°C for 2 or 4 h. Representative ginsenosides in the unsteamed and steamed extracts were determined using HPLC. The antiproliferative effects of the ginsenosides Rb1, Rg3 and Rh2 on SW-480 cells were determined by the MTS method. The effect of extract steamed for 4 h on apoptosis of SW-480 cell was assayed by flow cytometry after staining with annexin V/PI. The expression of 84 apoptotic-related genes, including TNF, mitochondria and p53 pathways, was determined using real-time quantitative PCR array analysis. The mitochondrial membrane potential (Δψm) was analyzed after staining with FC-1. Steaming of American ginseng increased Rg3 and Rh2 content and antiproliferative activity significantly. The quantitative PCR array data demonstrated that multiple genes in mitochondrial pathway are involved in American ginseng-induced apoptosis of SW-480 cells and the expression profiling was validated by the cellular functional assay. The mitochondrial pathway may play a key role in American ginseng-mediated cancer cell apoptosis.
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DOI：
10.1016/j.biocel.2006.10.005
发表时间：
2007-01-01
影响因子：
4
作者：
Cretney, Erika;Takeda, Kazuyoshi;Smyth, Mark J.
通讯作者：
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影响因子：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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