A hybrid resistive pulse-optical detection platform for microfluidic experiments.
A hybrid resistive pulse-optical detection platform for microfluidic experiments.
复制标题
DOI：
10.1038/s41598-017-10000-1
复制
发表时间：
2017-08-31
影响因子：
4.6
通讯作者：
Siwy ZS
中科院分区：
文献类型：
--
作者：
Hinkle P;Westerhof TM;Qiu Y;Mallin DJ;Wallace ML;Nelson EL;Taborek P;Siwy ZS
电阻脉冲传感是一种无标记方法，用于在单个颗粒通过离子传导通道或孔隙时对其进行表征。在电阻脉冲实验期间，当悬浮在溶液中的颗粒通过通道移位时，监测通过传导通道的离子电流。易位或“脉冲”过程中电流减小的幅度不仅取决于粒子和通道尺寸的比率，还取决于粒子的位置，而仅用电阻脉冲信号很难解决这一问题。我们提出了同时对通过微流体通道的粒子进行电学和光学检测的实验，以解决电阻脉冲的位置依赖性。在两个信号中同时跟踪粒子，以在同一时刻创建粒子位置到电阻脉冲幅度的映射。混合方法将提高对象表征的准确性，并为观察对象的动态变化（例如变形或方向变化）铺平道路。这种光学检测和电阻脉冲传感的组合方法将与其他混合高通量检测技术（例如成像流式细胞术）的尝试结合起来。
Resistive-pulse sensing is a label-free method for characterizing individual particles as they pass through ion-conducting channels or pores. During a resistive pulse experiment, the ionic current through a conducting channel is monitored as particles suspended in the solution translocate through the channel. The amplitude of the current decrease during a translocation, or ‘pulse’, depends not only on the ratio of the particle and channel sizes, but also on the particle position, which is difficult to resolve with the resistive pulse signal alone. We present experiments of simultaneous electrical and optical detection of particles passing through microfluidic channels to resolve the positional dependencies of the resistive pulses. Particles were tracked simultaneously in the two signals to create a mapping of the particle position to resistive pulse amplitude at the same instant in time. The hybrid approach will improve the accuracy of object characterization and will pave the way for observing dynamic changes of the objects such as deformation or change in orientation. This combined approach of optical detection and resistive pulse sensing will join with other attempts at hybridizing high-throughput detection techniques such as imaging flow cytometry.
登录
查看更多内容
影响因子：
6.1
作者：
Han Y;Gu Y;Zhang AC;Lo YH
通讯作者：
Lo YH
影响因子：
1.6
作者：
BERGE, LI;FEDER, J;JOSSANG, T
通讯作者：
JOSSANG, T
影响因子：
1.6
作者：
DEBLOIS, RW;BEAN, CP
通讯作者：
BEAN, CP
影响因子：
3.7
作者：
Lan, Wen-Jie;Kubeil, Clemens;White, Henry S.
通讯作者：
White, Henry S.
影响因子：
17.1
作者：
Pevarnik, Matthew;Schiel, Matthew;Siwy, Zuzanna S.
通讯作者：
Siwy, Zuzanna S.