Establishment of three novel cell lines derived from African American patients with colorectal carcinoma: A unique tool for assessing racial health disparity.
Establishment of three novel cell lines derived from African American patients with colorectal carcinoma: A unique tool for assessing racial health disparity.
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DOI：
10.3892/ijo.2018.4510
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发表时间：
2018-10
影响因子：
5.2
通讯作者：
Williams JL
中科院分区：
文献类型：
--
作者：
Paredes J;Ji P;Lacomb JF;Shroyer KR;Martello LA;Williams JL
非洲裔美国人（AAs）的结直肠癌（CRC）发病率和死亡率高于白人美国人（CAs）。为了评估与种族健康差异相关的分子特性，我们建立了来自3名AA受试者的结直肠肿瘤的3个细胞系。采用标准技术对CHTN06、SB501和SB521细胞系进行细胞和分子表征，包括免疫荧光、电镜、核型、逆转录聚合酶链反应、ELISA和免疫印迹分析。采用苏木精染色和伊红染色对CHTN06异种移植物进行组织学和形态学观察。共建立了3株源自原发肿瘤的AA CRC细胞系，并对其进行了鉴定。这些细胞系在没有永生化的情况下成功培养，并发现与小鼠异种移植一样具有致瘤性。在本研究中，免疫印迹和免疫荧光证实了CRC中已知的失调蛋白的表达，如p53、DNA错配修复蛋白和villin-1。与CA CRC细胞系（HT-29、HCT116和SW480）相比，AA CRC细胞系中致癌mirna（即miR-17、miR-21、miR-182、miR-210和miR-222）过表达。此外，与HT-29 （CA CRC细胞系）相比，AA CRC细胞系表现出不同的炎症谱；特别值得注意的是IL-8的分泌对炎症刺激的反应。总之，从AA CRC组织中获得了三个新的细胞系。这些细胞系的特点是上皮性质，与市售的CA来源细胞系相比，表现出几种mirna的差异表达和炎症反应。结直肠癌细胞系CHTN06、SB501和SB521代表了新的工具，可用于提供多种体外和体内模型来研究结直肠癌和种族健康差异。
The incidence and mortality rates of colorectal carcinoma (CRC) are higher among African Americans (AAs) compared with Caucasian Americans (CAs). To assess the molecular properties associated with racial health disparity, three cell lines derived from colorectal tumors of three AA subjects were established. Cellular and molecular characterization of the cell lines designated CHTN06, SB501 and SB521 was performed using standard technologies, including immunofluorescence, electron microscopy, karyotyping, reverse transcription-polymerase chain reaction, ELISA and immunoblot analysis. The histology and morphology of CHTN06 xenografts were examined by hematoxylin and eosin staining. A total of three AA CRC cell lines derived from primary tumors were established and characterized. These cell lines were successfully cultured without immortalization and were found to be tumorigenic as mouse xenografts. In the present study, immunoblotting and immunofluorescence confirmed the expression of proteins known to be dysregulated in CRC, such as p53, DNA mismatch repair proteins and villin-1. Oncogenic miRNAs (i.e., miR-17, miR-21, miR-182, miR-210 and miR-222) were overexpressed in the AA CRC lines compared with the CA CRC lines (HT-29, HCT116 and SW480). Additionally, the AA CRC cell lines exhibited a differential inflammatory profile compared with HT-29 (CA CRC cell line); specifically noted was IL-8 secretion in response to inflammatory stimuli. In conclusion, three novel cell lines derived from AA CRC tissues were generated. These cell lines were characterized as epithelial in nature and exhibited differential expression of several miRNAs and inflammatory responses compared with commercially available cell lines of CA origin. The CRC cell lines CHTN06, SB501 and SB521 represent novel tools that may be used to provide diverse in vitro and in vivo models for studying CRC and racial health disparity.
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影响因子：
168.9
作者：
Burn, John;Gerdes, Anne-Marie;Macrae, Finlay;Mecklin, Jukka-Pekka;Moeslein, Gabriela;Olschwang, Sylviane;Eccles, Diane;Evans, D. Gareth;Maher, Eamonn R.;Bertario, Lucio;Bisgaard, Marie-Luise;Dunlop, Malcolm G.;Ho, Judy W. C.;Hodgson, Shirley V.;Lindblom, Annika;Lubinski, Jan;Morrison, Patrick J.;Murday, Victoria;Ramesar, Raj;Side, Lucy;Scott, Rodney J.;Thomas, Huw J. W.;Vasen, Hans F.;Barker, Gail;Crawford, Gillian;Elliott, Faye;Movahedi, Mohammad;Pylvanainen, Kirsi;Wijnen, Juul T.;Fodde, Riccardo;Lynch, Henry T.;Mathers, John C.;Bishop, D. Timothy
通讯作者：
Bishop, D. Timothy
DOI：
10.1097/ppo.0b013e318250c001
发表时间：
2012-05
期刊：
Cancer journal (Sudbury, Mass.)
影响因子：
--
作者：
Iorio MV;Croce CM
通讯作者：
Croce CM
DOI：
10.1093/jnci/87.22.1686
发表时间：
1995-11-15
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
MAYBERRY, RM;COATES, RJ;EDWARDS, BK
通讯作者：
EDWARDS, BK
影响因子：
3.5
作者：
Puvvada, Soham D.;Funkhouser, William K.;O'Neil, Bert H.
通讯作者：
O'Neil, Bert H.
影响因子：
120.7
作者：
Lieberman, David A.;Holub, Jennifer L.;Morris, Cynthia D.
通讯作者：
Morris, Cynthia D.