RFX2 is broadly required for ciliogenesis during vertebrate development.
RFX2 is broadly required for ciliogenesis during vertebrate development.
复制标题
DOI：
10.1016/j.ydbio.2011.12.029
复制
发表时间：
2012-03-01
影响因子：
2.7
通讯作者：
Wallingford JB
中科院分区：
文献类型：
--
作者：
Chung MI;Peyrot SM;LeBoeuf S;Park TJ;McGary KL;Marcotte EM;Wallingford JB
在秀丽隐杆线虫中，RFX（Daf19）转录因子是纤毛发生的主要调节因子，控制制造纤毛所需的许多必需基因的表达。然而，在脊椎动物中，已经确定了七个RFX基因。生物信息学分析表明，Rfx2是Daf19最接近的同源物之一。因此，我们假设，Rfx2广泛控制脊椎动物发育过程中的纤毛发生。事实上，在这里，我们表明，Rfx2在非洲爪蟾的表达优先纤毛组织，包括神经管，胃腔顶板，表皮多纤毛细胞，耳泡，和肾脏。Rfx2基因敲低导致纤毛缺陷的胚胎表型，并且在Rfx2变体中观察到更少或截短的纤毛。这些结果表明Rfx2是脊椎动物纤毛发生所广泛需要的。此外，我们表明，Rfx2是必不可少的几个ciliogenic基因的表达，包括TTC 25，这是我们在这里显示所需的ciliogenesis，HH信号，和左右图案。
In Caenorhabditis elegans, the RFX (Daf19) transcription factor is a major regulator of ciliogenesis, controlling the expression of the many essential genes required for making cilia. In vertebrates, however, seven RFX genes have been identified. Bioinformatic analysis suggests that Rfx2 is among the closest homologues of Daf19. We therefore hypothesize that Rfx2 broadly controls ciliogenesis during vertebrate development. Indeed, here we show that Rfx2 in Xenopus is expressed preferentially in ciliated tissues, including neural tube, gastrocoel roof plate, epidermal multi-ciliated cells, otic vesicles, and kidneys. Knockdown of Rfx2 results in cilia-defective embryonic phenotypes and fewer or truncated cilia are observed in Rfx2 morphants. These results indicate that Rfx2 is broadly required for ciliogenesis in vertebrates. Furthermore, we show that Rfx2 is essential for expression of several ciliogenic genes, including TTC25, which we show here is required for ciliogenesis, HH signaling, and left–right patterning.
登录
查看更多内容
影响因子：
--
作者：
Goetz, Sarah C.;Ocbina, Polloneal J. R.;Anderson, Kathryn V.
通讯作者：
Anderson, Kathryn V.
影响因子：
3.6
作者：
Horvath, GC;Kistler, WS;Kistler, MK
通讯作者：
Kistler, MK
DOI：
10.1073/pnas.1005998107
发表时间：
2010-10-26
影响因子：
11.1
作者：
Hellman, Nathan E.;Liu, Yan;Drummond, Iain A.
通讯作者：
Drummond, Iain A.
影响因子：
64.8
作者：
Essner, JJ;Vogan, KJ;Brueckner, M
通讯作者：
Brueckner, M
DOI：
10.1002/ajmg.c.30231
发表时间：
2009-11-15
影响因子：
3.1
作者：
Baker, Kate;Beales, Philip L.
通讯作者：
Beales, Philip L.