Selection and identification of an RNA aptamer that specifically binds the HIV-1 capsid lattice and inhibits viral replication.
Selection and identification of an RNA aptamer that specifically binds the HIV-1 capsid lattice and inhibits viral replication.
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DOI：
10.1093/nar/gkab1293
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发表时间：
2022-02-22
影响因子：
14.9
通讯作者：
Lange MJ
中科院分区：
文献类型：
--
作者：
Gruenke PR;Aneja R;Welbourn S;Ukah OB;Sarafianos SG;Burke DH;Lange MJ
The HIV-1 capsid core participates in several replication processes. The mature capsid core is a lattice composed of capsid (CA) monomers thought to assemble first into CA dimers, then into ∼250 CA hexamers and 12 CA pentamers. CA assembly requires conformational flexibility of each unit, resulting in the presence of unique, solvent-accessible surfaces. Significant advances have improved our understanding of the roles of the capsid core in replication; however, the contributions of individual CA assembly forms remain unclear and there are limited tools available to evaluate these forms in vivo. Here, we have selected aptamers that bind CA lattice tubes. We describe aptamer CA15-2, which selectively binds CA lattice, but not CA monomer or CA hexamer, suggesting that it targets an interface present and accessible only on CA lattice. CA15-2 does not compete with PF74 for binding, indicating that it likely binds a non-overlapping site. Furthermore, CA15-2 inhibits HIV-1 replication when expressed in virus producer cells, but not target cells, suggesting that it binds a biologically-relevant site during virus production that is either not accessible during post-entry replication steps or is accessible but unaltered by aptamer binding. Importantly, CA15-2 represents the first aptamer that specifically recognizes the HIV-1 CA lattice.
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DOI：
10.1038/nrmicro3503
发表时间：
2015-08
期刊：
Nature reviews. Microbiology
影响因子：
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作者：
Campbell EM;Hope TJ
通讯作者：
Hope TJ
DOI：
10.1073/pnas.1419945112
发表时间：
2014-12-30
影响因子：
11.1
作者：
Bhattacharya, Akash;Alam, Steven L.;Yeager, Mark
通讯作者：
Yeager, Mark
DOI：
10.3390/v12111234
发表时间：
2020-10-30
期刊：
Viruses
影响因子：
--
作者：
Francis AC;Marin M;Prellberg MJ;Palermino-Rowland K;Melikyan GB
通讯作者：
Melikyan GB
影响因子：
--
作者：
Ganser-Pornillos BK;Yeager M;Pornillos O
通讯作者：
Pornillos O
DOI：
10.3390/v13061178
发表时间：
2021-06-20
期刊：
Viruses
影响因子：
--
作者：
Blanco-Rodriguez G;Di Nunzio F
通讯作者：
Di Nunzio F